Photosynthetic Processes and Mechanisms

New insights into the involvement of residue D1/V185 in Photosystem II function in Synechocystis 6803 and Thermosynechococcus vestitus.

This study investigates the effects of D1-V185T and D1-V185N mutations on PSII function in Synechocystis 6803 and Thermosynechococcus vestitus using EPR and optical kinetics, revealing altered proton transfer dynamics and water binding.

Integrative physiological plasticity of Agastache rugosa (Fisch. & C.A.Mey.) Kuntze reveals complex adaptation to light and nutrient gradients

This study examines how Agastache rugosa adapts to light and nutrient gradients through physiological and morphological changes, revealing unexpected patterns in Rubisco dynamics and nutrient use efficiency.

mRNA localization and thylakoid protein biogenesis in the filamentous heterocyst-forming cyanobacterium Anabaena sp. PCC 7120

The paper investigates mRNA localization and thylakoid protein biogenesis in Anabaena sp. PCC 7120, identifying an RNA-binding protein crucial for thylakoid protein translation and assembly.

Architecture of the ATP-driven motor for protein import into chloroplasts

The paper reports the 3.2-Å resolution structure of the Orf2971-FtsHi complex in Chlamydomonas reinhardtii, revealing its architecture and potential role in ATP-driven protein import into chloroplasts.

Unraveling the Evolutionary Trajectory of LHCI in Red-Lineage Algae: Conservation, Diversification, and Neolocalization

This study investigates the evolutionary changes in LHCI structures across red-lineage algae, identifying conserved and diversified Lhcr groups and introducing the concept of neolocalization.

Quantification of electron transport-related oxidative signals by time and wavelength-resolved redox biosensors and chlorophyll fluorescence

The paper quantifies redox signals and photosynthetic activity in potato plants using time and wavelength-resolved fluorescence spectra, revealing a link between electron transport rate and oxidative signals.

New evidence for the presence and function of phosphoinositides (PPIs) in the chloroplast

This study develops chloroplast-targeted biosensors to detect phosphoinositides (PPIs) and shows their presence, interactions, and role in stress responses in plants.

Uncovering the roles of the scaffolding protein CsoS2 in mediating the assembly and shape of the α-carboxysome shell

This study investigates the role of CsoS2-M in carboxysome shell assembly and shaping, revealing its function in enhancing shell protein association and recruiting CsoS2A for Rubisco packaging.

OsWHY1/OsTRXz/OsMORFs complex is essential for RNA modification and early chloroplast development in rice

This study identifies OsWHY1's role in chloroplast development by interacting with OsTRXz and OsMORFs to regulate RNA modification and maintain chloroplast stability.

Structural basis for molecular assembly of fucoxanthin chlorophyll a/c-binding proteins in a diatom photosystem I supercomplex

The paper determines the cryo-EM structure of a diatom PSI-FCPI supercomplex, identifies FCPI subunits, and reveals protein-protein interactions and evolutionary conservation of binding motifs.

In vivo ElectroChromic Shift measurements of photosynthetic activity in far-red absorbing cyanobacteria

This study adapts ElectroChromic Shift measurements to far-red absorbing cyanobacteria to assess photosynthetic efficiency and light energy conversion.

Biogenesis, engineering and function of membranes in the CO2-fixing pyrenoid

This study identifies proteins involved in pyrenoid membrane biogenesis and shows their role in CO2 delivery, with implications for engineering functional pyrenoids in crops.

Molecular events accompanying aggregation-induced energy quenching in Fucoxanthin-Chlorophyll Proteins

This study investigates molecular changes in Fucoxanthin-Chlorophyll Proteins (FCPs) during aggregation-induced energy quenching in diatoms, revealing structure-dependent conformational shifts linked to non-photochemical quenching.

In vivo analysis of the relationship between CP26 and qE-type NPQ via higher-order Arabidopsis cp26 mutants

This study investigates the role of CP26 in NPQ regulation in Arabidopsis by analyzing higher-order cp26 mutants and shows that NPQ deficiency in these mutants is independent of qE and qZ.

Functional Modification of Cyanobacterial Phycobiliprotein and Phycobilisomes through Bilin Metabolism Control

The paper engineers a cyanobacterial strain to control PEB metabolism, enabling reversible color shifts and structural changes in phycobilisomes for improved light harvesting.

PPR767 affects plant architecture and drought resistance by modulating complex I activity and ROS content in rice

This study identifies PPR767's role in rice by modulating mitochondrial complex I activity and ROS content, affecting plant architecture and drought resistance.

Strong heterologous electron sink outcompetes alternative electron transport pathways in photosynthesis

The paper demonstrates that a heterologous electron sink outcompetes natural electron transport pathways in engineered cyanobacteria, revealing FNR as the preferred route for electron delivery and highlighting the NADPH/NADP+ ratio's role in electron flux.

Two mechanisms of photoenergy regulation revealed by kinetic behaviors of chlorophyll fluorescence during light adaptation

The paper identifies two mechanisms of photoenergy regulation in PSII using chlorophyll fluorescence kinetics: one involving PsbS-mediated NPQ and another involving zeaxanthin and lutein disrupting energy transfer to P680.

The role of chloroplast SRP54 domains and its C-terminal tail region in post- and cotranslational protein transport in vivo

This study investigates the role of cpSRP54 domains and its C-terminal tail in chloroplast protein transport, showing their importance in post- and cotranslational transport of photosynthetic proteins.

Conserved and repetitive motifs in an intrinsically disordered protein drive α-carboxysome assembly

This study identifies conserved and repetitive motifs in CsoS2 that drive α-carboxysome assembly and demonstrates in vitro reconstitution of carboxysomes.

A system biology-oriented investigation of Arabidopsis proteomes altered in chloroplast biogenesis and retrograde signaling reveals adaptive responses at whole cell level.

The paper investigates Arabidopsis proteome changes in chloroplast biogenesis and retrograde signaling, identifying adaptive responses and a new role for PsbO.

Cryo-EM insight into hydrogen positions and water networks in photosystem II

Cryo-EM structure of PSII at 1.71 [A] resolution reveals over 50% of hydrogen and proton sites and additional water binding sites, aiding catalytic insight.

Systematic exploration of bacterial form I rubisco maximal carboxylation rates

This study characterizes the kinetic properties of over 100 bacterial form I rubiscos to identify factors influencing carboxylation rates, finding that carboxysomes and cyanobacterial origins are associated with faster rates.

Sulfoquinovosyl diacylglycerol is required for dimerization of the Rhodobacter sphaeroides RC-LH1 core complex

This study identifies sulfoquinovosyl diacylglycerol (SQDG) as essential for dimerization of the RC-LH1 complex in Rhodobacter sphaeroides, but not for its monomeric function.

Realisation of a key step in the evolution of C4 photosynthesis in rice by genome editing.

This paper uses genome editing to relocalize carbonic anhydrase activity in rice, achieving a key step in C4 photosynthesis evolution and revealing a new role for chloroplast CA in photosynthetic induction.

A Genetically Encoded System to Quantify and Evolve RuBisCO-Catalyzed Carbon Fixation

This paper develops a genetically encoded system to quantify and evolve RuBisCO-catalyzed CO2 fixation in bacteria, showing agreement with in vitro kinetics and enabling enzyme evolution.

Advancing chloroplast synthetic biology through high-throughput plastome engineering of Chlamydomonas reinhardtii

This study establishes Chlamydomonas reinhardtii as a platform for chloroplast synthetic biology, enabling high-throughput engineering of plastomes and demonstrating improved biomass production through synthetic pathways.

The major nucleoid-associated protein WHIRLY1 promotes chloroplast development in barley

This study characterizes the barley WHIRLY1 mutant, showing it promotes chloroplast development by remodeling nucleoids and coordinating protein expression, but is not essential for chloroplast biogenesis.

Isolation, cloning and expression analysis of two pyruvate orthophosphate dikinase genes of Chlamydomonas reinhardtii

The paper isolates and clones two PPDK genes in C. reinhardtii, showing they encode functional enzymes responsive to CO2 and ammonium, and are evolutionarily closer to plant PPDKs.

Synchronization between chloroplastic and cytosolic protein synthesis for photosynthesis complex assembly

The paper investigates the coordination of chloroplast and cytoplasmic protein synthesis during light/dark transitions in plants, revealing translational control in chloroplasts and mRNA abundance control in the cytoplasm.