Bacterial biofilms and quorum sensing

Environmental sensing in dynamic quorum responses

The paper shows quorum sensing in bacteria can detect environmental changes and uses a new signaling mechanism involving protein synthesis and dilution rates to process dynamic inputs.

Metabolic activity affects response of single cells to a nutrient switch in structured populations

This study shows that metabolic activity variation in structured microbial populations affects single-cell responses to nutrient switches, with cross-feeding cells having higher growth resumption probability.

Engineered orthogonal quorum sensing systems for synthetic gene regulation

The paper identifies orthogonal HSL-synthase-regulator pairs that minimize crosstalk in synthetic gene regulation systems in E. coli.

On a Robust, Sensitive Cell-Free Method for Pseudomonas Sensing and Quantification

The paper presents a cell-free method for sensing and quantifying the quorum sensing molecule 3OC12HSL in Pseudomonas infections using droplet microfluidics.

Interaction of N-3-oxododecanoyl homoserine lactone with transcriptional regulator LasR of Pseudomonas aeruginosa: Insights from molecular docking and dynamics simulations

The paper analyzes the molecular interactions of the autoinducer 3-0-C12-HSL with the LasR protein of Pseudomonas aeruginosa using docking and dynamics simulations, identifying a new interaction site and dimerization form.

Mechanistic insights of the attenuation of quorum-sensing-dependent virulence factors of Pseudomonas aeruginosa: Molecular modeling of the interaction of taxifolin with transcriptional regulator LasR

This study investigates how taxifolin interacts with the LasR transcriptional regulator of Pseudomonas aeruginosa using molecular docking, dynamics simulations, and machine learning to identify two binding modes.

A global genomic approach uncovers novel components for twitching motility-mediated biofilm expansion in Pseudomonas aeruginosa

The paper uses TraDIS to identify genes involved in twitching motility-mediated biofilm expansion in Pseudomonas aeruginosa, revealing both known and novel components.

Characterization of Diverse Homoserine Lactone Synthases in Escherichia coli

This study characterizes ten homoserine lactone synthases in E. coli, showing their compatibility with the Lux pathway and highlighting disposal risks of HSLs.

A synthetic bacterial information transfer system functions in the mammalian gut

The paper engineers a synthetic bacterial communication system in the mammalian gut using quorum sensing and bacteriophage genetic switch circuits.

Tools for engineering coordinated system behaviour in synthetic microbial consortia

The paper develops a library of AHL-receiver devices and a software tool to identify orthogonal chemical communication channels for synthetic microbial consortia.

Cheating on cheaters dramatically affects social interactions in Pseudomonas aeruginosa

When pyoverdine and elastase are both needed, a pvdS mutant outcompetes the lasR cheater in P. aeruginosa and averts population collapse; a cost-ratio model with quorum sensing explains this.

Biofilm Lithography: High-resolution cell patterning via optogenetic adhesin expression

This paper introduces Biofilm Lithography, a method to create high-resolution structured biofilms using optogenetic adhesin expression, achieving spatial resolution down to 25 micrometers.

Interaction of quercetin with transcriptional regulator LasR of Pseudomonas aeruginosa: Mechanistic insights of the inhibition of virulence through quorum sensing

This study investigates how quercetin interacts with LasR, a transcriptional regulator in Pseudomonas aeruginosa, to inhibit quorum sensing and biofilm formation through two distinct binding modes.

Length and time scales of cell-cell signaling circuits in agar

The paper investigates how cell-cell communication circuits operate in a heterogeneous environment, finding that signal diffusion and colony placement affect communication range and speed.

Cell-free and in vivo characterization of Lux, Las, and Rpa quorum activation systems in E. coli

The paper characterizes three quorum sensing systems in E. coli using cell-free and in vivo methods, finding significant genetic and signal crosstalk.