Ubiquitin and proteasome pathways

Site-specific K63 ubiquitinomics reveals post-initiation regulation of ribosomes under oxidative stress

The paper identifies K63 ubiquitination sites on ribosomes in yeast under oxidative stress using mass spectrometry, revealing their role in post-initiation translation control.

Structures of the substrate-engaged 26S proteasome reveal the mechanisms for ATP hydrolysis-driven translocation

The paper presents cryo-EM structures of the 26S proteasome in four conformational states to elucidate how ATP hydrolysis drives substrate translocation and deubiquitination.

Mitotic replisome disassembly in vertebrates

The paper shows that mitotic replisome disassembly in vertebrates depends on TRAIP-mediated ubiquitylation of Mcm7 and p97/VCP activity, and is conserved across higher eukaryotes.

USP21 and OTUD3 Antagonize Regulatory Ribosomal Ubiquitylation and Ribosome-Associated Quality Control

The paper identifies USP21 and OTUD3 as deubiquitylating enzymes that counteract ZNF598-mediated ribosomal ubiquitylation, facilitating ribosome-associated quality control.

Targeted substrate degradation by Kelch controls the actin cytoskeleton during ring canal expansion

The paper identifies HtsRC as a substrate of the CRL3Kelch ubiquitin ligase complex, showing its role in ring canal expansion through proteasomal degradation.

Trade-off and flexibility in the dynamic regulation of the cullin-RING ubiquitin ligase repertoire

The paper uses mathematical modeling to show that Cand1 exchange activity creates a trade-off between ligase activity and receptor exchange, optimizing substrate degradation and enabling flexible CRL repertoire adjustment.

An "invisible" ubiquitin conformation is required for efficient phosphorylation by PINK1

The paper shows that an 'invisible' conformation of ubiquitin enhances its phosphorylation by PINK1 through a dynamic equilibrium involving the Ser65 loop.

A 20S proteasome receptor for degradation of intrinsically disordered proteins

The paper identifies PSMA3 as a receptor for 20S proteasome-mediated degradation of intrinsically disordered proteins (IDPs) and shows its C-terminal fragment inhibits IDP degradation.

iSUMO - integrative prediction of functionally relevant SUMOylation events

iSUMO integrates sequence and protein features to predict functional SUMOylation events, identifying more targets in human and yeast than existing tools at the same false positive rate.

Integrative meta-analysis reveals that most yeast proteins are very stable

The paper re-analyzes protein half-life data in yeast to provide consistent estimates, finding that protein stability correlates with abundance and function.

A SUMO-Ubiquitin Relay Recruits Proteasomes to Chromosome Axes to Regulate Meiotic Recombination

The paper shows that SUMO and ubiquitin-proteasome systems regulate meiotic prophase events in mouse by controlling protein turnover through RNF212 and HEI10.