Insect Resistance and Genetics

Genome sequence and efficient CRISPR/Cas9 gene editing in the solanacea specialist pest Tetranychus evansi

This paper presents a high-quality genome assembly and CRISPR/Cas9 gene editing system for Tetranychus evansi, enabling studies of pesticide resistance and host adaptation.

Metabolic resistance of the tiger mosquito to pyrethroid insecticides in La Reunion Island likely results from local adaptation

This study identifies genetic mechanisms of pyrethroid resistance in La Reunion Island tiger mosquitoes, showing it results from local adaptation through detoxification gene over-expression and a large inverted superlocus.

Genome-wide association studies identify new candidate genes and tissues underlying resistance to a natural toxin in drosophilids

This study identifies new candidate genes and tissues associated with resistance to a natural toxin in Drosophila species through genome-wide association and transcriptome analyses.

A draft genome assembly of the agricultural pest Leucoptera coffeella and analysis of its dsRNA processing machinery is a key step towards RNAi-based biopesticides in Lepidoptera

The paper presents a draft genome assembly of Leucoptera coffeella and identifies genes involved in dsRNA processing, supporting RNAi-based biopesticide development.

A panel of CYP6Z genes drives broad-spectrum cross-resistance to public health insecticides in the major malaria vector An. gambiae s.s.

This study identifies CYP6Z genes in An. gambiae as drivers of broad-spectrum insecticide resistance through transcriptional upregulation and functional validation.

Evolving resistance patterns in Tetranychus urticae and Bemisia tabaci in Greece

This study assesses pesticide resistance in Tetranychus urticae and Bemisia tabaci in Greece using bioassays and ddPCR, revealing widespread resistance mutations and highlighting the need for integrated pest management.

New genomic resources for an invasive mite pest expand the known diversity of pesticide resistance mechanisms and guide development of KASP diagnostic markers

This paper presents a chromosome-level genome and genomic dataset for the invasive mite Halotydeus destructor, identifies pesticide resistance mutations, and develops KASP markers for resistance diagnostics.

Divergent evolution of insecticidal protein families across bacteria and ferns

The paper investigates the evolutionary origins of insecticidal protein families, finding evidence of divergent evolution and horizontal gene transfer across bacteria, ferns, and other organisms.

A Grapholita molesta (Lepidoptera: Tortricidae) cadherin in the 99C cadherin clade binds to Bacillus thuringiensis Cry1A proteins

This study identifies and validates GmCad1 as a Cry1A receptor in Grapholita molesta, showing its binding to Cry1Aa, Cry1Ab, and Cry1Ac proteins.

Bulk segregant analysis reveals genomic regions associated with imidacloprid resistance in the Colorado potato beetle

The paper uses bulk segregant analysis to identify genomic regions linked to imidacloprid resistance in Colorado potato beetles and finds that RNAi reduces resistance, challenging its use for validation.

Lack of tolerance development following sublethal Cry1 proteins exposure in Spodoptera exigua

This study investigates whether sublethal exposure to Cry1 proteins in Spodoptera exigua larvae increases tolerance, finding no significant development of tolerance in larvae or their offspring.

MgAl-layered double hydroxide nanoparticles mediated oral delivery of dsRNA silencing jhp and chi genes with sublethal effects in Phthorimaea absoluta

This paper evaluates MgAl-LDH nanoparticles as a dsRNA delivery system for targeting jhp and chi genes in Phthorimaea absoluta, showing sublethal effects and gene expression reduction.

UGT35B1 is the principal enzyme mediating nicotine glycosylation in Drosophila melanogaster

This study identifies Ugt35B1 as the key enzyme for nicotine glycosylation in Drosophila, revealing an insect detoxification pathway.

Spread of a single superclone drives insecticide resistance in Acyrthosiphon kondoi across an invasive range

The paper identifies a superclone driving insecticide resistance in Acyrthosiphon kondoi across its invasive range through genomic and functional analyses.

Functional loss of CHS2 confers high levels resistance to Bacillus thuringiensis Vip3Aa in Spodoptera exigua and Agrotis ipsilon

This study identifies CHS2 gene knockout as a mechanism conferring high resistance to Vip3Aa in Spodoptera exigua and Agrotis ipsilon.

Double-stranded DNA reduces dsRNA degradation in the saliva and significantly enhanced RNAi-mediated gene silencing in Halyomorpha halys

This study shows that dsDNA enhances dsRNA stability in Halyomorpha halys saliva, improving RNAi-mediated gene silencing but not significantly increasing mortality when delivered orally.

Transgenic Cowpea Expressing Synthetic BtCry1Ab Confers High Resistance to Legume Pod Borer (Maruca vitrata)

This study develops transgenic cowpea expressing synthetic BtCry1Ab, showing high resistance to legume pod borer and other lepidopteran pests without affecting growth or producing new metabolites.

Recurrent Mutations Drive the Rapid Evolution of Pesticide Resistance in the Two-spotted Spider Mite Tetranychus urticae

Ribosomal RNA-specific antisense DNA and double-stranded DNA trigger rRNA biogenesis and insecticidal effect on insect pest Coccus hesperidum L.

This paper investigates the insecticidal potential of short and long antisense DNA oligos against Coccus hesperidum, finding that short 11-mer oligos achieve higher mortality rates than longer sequences.

The cys-loop ligand-gated ion channel gene superfamily of the Colorado potato beetle, Leptinotarsa decemlineata

The paper identifies and characterizes 22 cys-loop ligand-gated ion channel subunit genes in the Colorado potato beetle, including novel duplications and RNA editing events.

Over-expression and increased copy numbers of a cytochrome P450 and two UDP-glucuronosyltransferase genes in macrocyclic lactone resistant Psoroptes ovis of cattle

The paper identifies over-expression and copy number variation of cytochrome P450 and UDP-glucuronosyltransferase genes in ML-resistant Psoroptes ovis mites from cattle.

Refined CRISPR/Cas9 genome editing in pea aphids uncovers the essential roles of Laccase2 in overwintering egg adaptation

This study uses CRISPR/Cas9 to knockout Laccase2 in pea aphids, revealing its essential role in eggshell pigmentation, hardening, and survival during overwintering diapause.

Mechanistic evidence of widespread insecticide resistance among Illinois West Nile virus vectors (Culex pipiens and Culex restuans)

The paper assesses insecticide resistance in West Nile virus vectors in Illinois, finding variation in resistance mechanisms across species and regions.

Exploring the connections between digestion and detoxification in microevolution of insecticide resistance of the tea tortrix moth, Adoxophyes honmai

This study examines the microevolutionary link between digestion and insecticide resistance in tea tortrix moths, finding constitutively higher digestive gene expression in resistant larvae.

Knockdown-resistance (kdr) mutations in Indian Aedes aegypti populations: lack of recombination among haplotypes bearing V1016G, F1534C, and F1534L kdr alleles

The paper identifies five kdr mutations in Indian Ae. aegypti populations and finds no recombination among haplotypes with V1016G, F1534C, and F1534L alleles.

Identification of a novel resistance gene which provides insight into Vip3Aa mode of action in Helicoverpa armigera

This study identifies a novel gene, HaVipR1, responsible for Vip3Aa resistance in Helicoverpa armigera through genetic crosses and genomic analysis, showing its role in conferring resistance independently of known genes.

Pleiotropic functions of an antenna-specific odorant binding protein linking xenobiotic adaptation and olfaction in the Colorado potato beetle, Leptinotarsa decemlineata

This study identifies and functionally characterizes an antenna-specific odorant binding protein in the Colorado potato beetle linked to insecticide resistance and host location.

Optimizing dsRNA sequences for RNAi in pest control and research with the dsRIP Web-Platform

This study identifies sequence features for effective dsRNA in insect pest control and develops a web-platform to optimize dsRNA design for RNAi.

Chronic dietary ingestion of Bacillus thuringiensis spores promotes intestinal inflammation and aging in non-target adult Drosophila

This study shows chronic Bt spore ingestion in Drosophila reduces lifespan, causes gut inflammation, and promotes aging.

RNA degradomics and proteomics reveal the mechanism of dsProsβ1-mediated proteasome targeting in the cabbage stem flea beetle

This paper investigates dsProsβ1-mediated proteasome targeting in the cabbage stem flea beetle using RNA degradomics and proteomics to reveal molecular mechanisms of RNAi-based pest control.