Zebrafish Biomedical Research Applications

This review meta-analyzes 38 studies on unpredictable chronic stress in zebrafish, finding increased anxiety/fear and cortisol, decreased locomotor function, but no significant effect on social behavior, while highlighting methodological issues.

This study shows that thyroid hormone affects zebrafish swimming mechanics by altering passive stiffness and kinematic patterns during development.

The paper develops a protocol to generate floxed alleles in zebrafish for cell-specific knockout, demonstrating its utility in studying gene function.

This study shows zebrafish lateral line hair cells detect serotonin, influencing navigation behavior in response to environmental cues.

The paper shows that zebrafish inpp5ka and inpp5kb, duplicated from whole genome duplication, have diverged in expression and function, with inpp5ka more closely related to human INPP5K and inpp5kb losing catalytic activity.

This study identifies stac1 in zebrafish embryos and shows it is required for sensory stimulus-induced escape locomotion via regulation of CSF-cNs.

This study shows that aged zebrafish and turquoise killifish exhibit reduced fin regeneration and angiogenesis, with VEGF-A expression and vascular density changes, suggesting a key role for angiogenesis in aging wound healing.

This study maps the connectivity of V2b neurons in larval zebrafish spinal circuits, showing they inhibit motor neurons and other interneurons, with patterned inhibition along the rostrocaudal axis.

The paper introduces zFACE, a method for analyzing craniofacial development in zebrafish embryos, showing that smarca4a loss causes craniofacial anomalies similar to human Coffin-Siris syndrome.

The paper shows that early pre-neural serotonin in zebrafish affects later serotonin synthesis and behavioral patterns without causing major developmental abnormalities.

This study examines the effects of valproic acid on zebrafish optic tectum development, showing impaired neuronal specification and neurite extension.

The paper identifies temporally and spatially distinct morphogenetic processes in zebrafish tail fin vascular development.

This paper introduces a method to image adult zebrafish brains using MRI for longitudinal studies, including a 3D-printed life support chamber and optimized contrast agent delivery.

This study shows zebrafish can re-vascularize spinal tissue after injury, with early angiogenesis and barrier restoration, but Vegfr2 inhibition does not block vessel formation.

PyZebrascope provides an open-source platform for whole-brain neural activity imaging in zebrafish using light-sheet microscopy, enabling high-throughput and stable data acquisition.

FRaeppli enables multispectral imaging for cell tracing and tissue analysis in zebrafish, revealing new canalicular topologies in hepatocytes.

The paper compares zebrafish Switch reporter transgenes to identify those with optimal recombination efficiency and expression patterns for lineage labeling.

This study investigates the functional effects of two CACNA1C mutations in zebrafish, showing altered mRNA levels, neurotransmitter profiles, and behavioral and electrophysiological changes linked to psychiatric disorders.

The paper identifies two subpallial neuronal populations in larval zebrafish that express molecular markers associated with mammalian striatal pathways and show developmental increases.

This study identifies 1408 genes and 661 proteins differentially regulated during zebrafish caudal fin regeneration using transcriptomics and proteomics.

This study investigates the in vivo roles of RHOA in vascular development and integrity using zebrafish models, finding that both gain- and loss-of-function lead to vascular dysfunction.

This study shows that dlc1 is essential for vascular and cardiac development in zebrafish embryos, functioning downstream of Vegfa/Kdrl/Nrp1 signaling and linked to congenital heart defects in humans.

The paper identifies invasive skin-derived cells in zebrafish lateral line that differentiate into ionocytes, adapting to environmental ionic changes and regulating hair cell function.

ZeBraInspector enables high-content 3D imaging and volumetric analysis of zebrafish brain white matter to characterize phenotypes and treatment effects.

The paper generates zebrafish CRISPR mutants for DNA repair genes and identifies their roles in development and disease.

This study shows morphine reduces pain after heart cryoinjury in zebrafish without hindering regeneration, while lidocaine delays repair.

The paper identifies acta1b as the best gene for modeling cardiac actin-related heart disease in zebrafish by analyzing the phenotypic effects of CRISPR targeting actc1a and acta1b.

This study shows that hapln1b, an ECM component, is required for HSC specification in zebrafish by modulating kit signaling and maintaining ECM integrity.

The paper shows that vascular injury leads to changes in vessel network topology to increase blood flow and activate Notch-ephrinb2 signaling for microcirculation restoration.

The paper combines multiple imaging techniques to detect cardiac defects in adult zebrafish that were previously undetectable.