Christian Mosimann

Rbm8a deficiency causes hematopoietic defects by modulating Wnt/PCP signaling

This study shows that rbm8a deficiency in zebrafish causes hematopoietic defects by impairing non-canonical Wnt/PCP signaling, leading to reduced thrombocytes and LPM anomalies.

Discovery of a primed endothelial progenitor that requires VEGF/ERK inhibition to complete vein differentiation

The paper identifies a primed endothelial progenitor that requires VEGF/ERK inhibition to differentiate into vein endothelial cells, revealing a two-step process for vein development.

The pericardium forms as a distinct structure during heart formation

This study identifies the pericardium's origin from anterior mesothelial progenitors in zebrafish, distinct from the heart field, and links pericardial stiffness to pediatric cardiomyopathies.

Genetic context of transgene insertion can influence neurodevelopment in zebrafish

The study investigates neurodevelopmental effects of a zebrafish Gal4 transgenic line, finding phenotypes linked to transgene insertion site rather than Gal4 toxicity.

Lineage labeling with zebrafish hand2 Cre and CreERT2 recombinase CRISPR knock-ins

This paper develops zebrafish hand2 Cre and CreERT2 drivers using CRISPR-Cas9 to label mesodermal and ectodermal lineages, revealing lineage contributions to various tissues and vasculature.

Quantification of subcellular RNA localization through direct detection of RNA oxidation

This paper introduces OINC-seq, a method to quantify subcellular RNA localization through direct oxidation detection, and demonstrates its application in various cellular compartments and live zebrafish.

CO-OPTION OF NECK MUSCLES SUPPORTED THE VERTEBRATE WATER-TO-LAND TRANSITION

This study reconstructs the mesodermal origins of neck muscles in vertebrates, showing that neck evolution arose from pre-existing muscle groups in fishes through developmental expansion.

Multiple embryonic sources converge to form the pectoral girdle skeleton in zebrafish

This study identifies the embryonic origins of the zebrafish pectoral girdle, revealing multiple developmental contributions and evolutionary implications for girdle transformation.

pIGLET: Safe harbor landing sites for reproducible and efficient transgenesis in zebrafish

The paper establishes phiC31 integrase-based transgene landing sites in zebrafish, enabling efficient and reproducible transgenesis with 25-50% germline transmission efficiency.

Conserved enhancer logic controls the notochord expression of vertebrate Brachyury

The paper identifies conserved notochord-specific enhancers of Brachyury/T/TBXT in vertebrates and shows their role in notochord expression and development.

Urp1 and Urp2 act redundantly to maintain spine shape in zebrafish larvae.

This study shows that Urp1 and Urp2 neuropeptides are redundant in maintaining spine shape in zebrafish larvae through myosin II-dependent mechanisms.

Next-generation plasmids for transgenesis in zebrafish and beyond

The paper introduces next-generation transgenesis tools for zebrafish and other species, including vectors with fluorescent markers and promoters for specific tissue expression.

The centrosomal protein 83 (CEP83) regulates human pluripotent stem cell differentiation towards the kidney lineage

This study shows that CEP83 deficiency in hiPSCs disrupts intermediate mesoderm differentiation, leading to impaired kidney progenitor formation and increased LPM marker expression.

Heterogeneity and genomic loci of ubiquitous Cre reporter transgenes in zebrafish

The paper compares zebrafish Switch reporter transgenes to identify those with optimal recombination efficiency and expression patterns for lineage labeling.

Definitive Hematopoietic Stem Cells Minimally Contribute to Embryonic Hematopoiesis

This study shows nascent hematopoietic stem cells (HSCs) self-renew but have delayed differentiation during embryonic hematopoiesis, with HSC-independent progenitors driving early blood development.

Persistent ventricle partitioning in the adult zebrafish heart

The paper investigates the contribution of first and second heart field lineages to the adult zebrafish ventricle, finding persistent cell-based compartmentalization without physical boundaries.

Hand2 delineates mesothelium progenitors and is reactivated in mesothelioma

This study identifies Hand2 as a key regulator of mesothelium progenitors in zebrafish and shows its reactivation in mesothelioma, linking developmental processes to disease.

A conserved regulatory program drives emergence of the lateral plate mesoderm

The paper identifies a conserved regulatory program driving lateral plate mesoderm emergence across chordates using zebrafish and comparative analysis.

Mutations in Bcl9 and Pygo genes cause congenital heart defects by tissue-specific perturbation of Wnt/β-catenin signaling

Cre/lox-controlled spatio-temporal perturbation of FGF signaling in zebrafish

This study develops a genetic tool to spatio-temporally perturb FGF signaling in zebrafish and demonstrates its use in studying cell-autonomous requirements for FGF signaling in pectoral fin development.

Continuous addition of progenitors forms the cardiac ventricle in zebrafish

This study tracks zebrafish heart development and shows that the ventricle forms continuously from progenitor cells, with FGF signaling playing a critical role in this process.

Highly efficient DNA-free gene disruption in the agricultural pest Ceratitis capitata by CRISPR-Cas9 RNPs

This paper demonstrates efficient CRISPR-Cas9-based gene disruption in the medfly using RNPs, achieving high germline transmission and mutant phenotypes.

CRISPR-Cas9 targeted disruption of the yellow ortholog in the housefly identifies the brown body locus

This study identifies the yellow ortholog as the brown body locus in houseflies using CRISPR-Cas9 and confirms its role in melanization.

Early frameshift alleles of zebrafish tbx5a that fail to develop the heartstrings phenotype

The paper generates two new zebrafish tbx5a frameshift alleles and compares their phenotypes to the classic hst mutant, finding differences in heart defects.

CrispRVariants: precisely charting the mutation spectrum in genome engineering experiments

CrispRVariants provides a toolkit to accurately assess mutagenesis efficiency in genome editing experiments by analyzing sequencing data and resolving mutant alleles.