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This study develops water-soluble fluorogenic probes for single-molecule glycosidase activity profiling in blood, revealing -mannosidase as a potential biomarker for liver injury.

The paper analyzes metabolomics 'dark matter' using a detectable khipu model, showing most abundant features have identifiable ion patterns, while many remain unknown.

This study shows dimeric Alix nucleates CHMP4 polymerization, essential for ESCRT-III membrane remodeling, and demonstrates that dimerization is required for plasma membrane repair.

This study investigates how PCH7-linked TOE1 mutations affect thermal stability, ribonuclease activity, and oligomerization, revealing their impact on TOE1 biochemistry.

This study investigates the phase separation and amyloid aggregation properties of 14-3-3 protein isoforms, finding that some forms can accelerate α-synuclein aggregation and form heterotypical droplets with α-synuclein.

This study identifies a complex lipid profile in Borrelia burgdorferi, revealing its reliance on environmental lipids and its ability to synthesize eukaryotic-like lipids.

This study examines how zinc and carbonate chemistry affect the carbon concentrating mechanism and growth of the diatom P. tricornutum.

The paper shows that measurement strategies affect how codon optimality influences translation versus mRNA decay, altering the perceived division of labor between these processes.

This study shows that cMLCK forms biomolecular condensates that enhance myosin motor phosphorylation in cardiac muscle cells, revealing a mechanism for regulating heart contractile function.

The paper challenges claims about oxygen resistance in CO dehydrogenase mutants by showing A559W and A559W/V610H variants are not more resistant than wild-type.

This study defines the mechanism of Ryanozole's Ca2+-dependent modulation of RyR2 using cryo-EM, mutagenesis, and functional assays, revealing its structural basis for CPVT therapy.

This study identifies the earliest co-transcriptional assembly intermediates of the eukaryotic 60S subunit and reveals the molecular logic of an assembly checkpoint.

This study shows that circadian disruption causes sex-specific changes in gut microbiota and bioactive lipid profiles, with distinct adaptations in males and females.

The paper identifies an AMPK-FLCN-TFE3 pathway that promotes hypoxia survival through lysosomal and mitochondrial biogenesis, with implications for cancer therapy.

This study identifies substrate specificity of bacterial ACS transporters using genetics, biochemistry, and structural biology, revealing structural determinants of stereoselectivity in LgoT.

The paper uses visual proteomics to identify functional spirosomes in Clostridium thermocellum during biomass degradation.

This paper identifies a novel compound targeting Mycobacterium tuberculosis cytochrome bd through in vitro screening and structural analysis, showing growth inhibition and competitive inhibition at the quinol-binding site.

The paper assesses how fungal selection methods and environment-mimicking culture conditions affect the diversity of natural product libraries.

This paper develops L-DNA nanocubes for targeted drug delivery, showing improved stability and therapeutic efficacy compared to D-DNA nanocubes.

The paper characterizes the yeast secretome under various proteosynthetic stress conditions and identifies BiP as a functional extracellular chaperone in specific stress scenarios.