Discover, rate, and discuss biology preprints.

The paper analyzes genomic variations in seven Bluebunch wheatgrass species to reveal genetic diversity, population structure, and evolutionary dynamics, identifying six genetic clusters and reticulate evolution in Eurasian species.

The paper analyzes bulk RNA-seq data from six primate cerebellar subregions to identify gene expression differences across species, highlighting divergence in synaptic activity and glucose metabolism in lateral hemispheres.

The paper compares pseudouridine modification sites in primary and immortalized human T cells using direct RNA sequencing, finding most sites are conserved with some cell-type specific differences.

The paper demonstrates a method using LSTM networks to generate dynamic omics data, showing it can predict biological responses with few samples and uncovering a paradoxical BrlA response to micafungin.

This study introduces DANcer, a deep learning classifier that identifies robust ALS molecular subtypes across bulk and single-cell datasets, showing they correlate with clinical features and cellular mechanisms.

The paper analyzes transposable elements in arbuscular mycorrhizal fungi to show their role in genome evolution and host interaction, finding parallels with plant pathogens.

This study uses single-cell RNA sequencing to show that clonal CHO-K1 cells exhibit subpopulation heterogeneity in production stability over time.

This study identifies differentially expressed genes and pathways in Tibetan sheep testicular tissue under hypoxia, suggesting their role in reproductive adaptation to high altitude.

This study uses HiF-snRNA-seq to analyze the cingulate cortex in Parkinson's disease and Parkinson's disease dementia, identifying cell-type-specific gene expression changes and disease-stage-related pathways.

This study shows that KRAB-zinc-finger proteins regulate endogenous retroviruses in the germline, shaping transcriptomes and genome evolution through evolutionary conserved mechanisms.

The paper presents a high-quality reference genome for the banded cricket and identifies genomic targets for selective breeding through comparative genomics.

The paper examines translational selection across 223 metazoan species, finding weak evidence of codon optimization in mammals and a correlation between population size and selection intensity.

This study maps quantitative trait loci for CSF metabolites and identifies associations with brain traits, revealing potential neurobiological pathways.

The paper identifies essential genes in porcine cell lines using CRISPR screening and compares them to human essential genes.

CARPDM enables cost-effective antibiotic resistome profiling in metagenomic samples through targeted enrichment and in-house probe synthesis.

CasGen generates novel Cas9 and Cas12 proteins using a transformer-based model with margin-based regularization, achieving structural similarity and functional integrity.

This study creates a single-cell RNA-seq atlas of the mouse seminal vesicle, identifying secretory and immune cell populations and their transcriptomes.

UniVI integrates multimodal single-cell data using a mixture-of-experts β-variational autoencoder, improving cross-modal alignment and label transfer across various data types and study designs.

The paper evaluates changes in glia-neuron communication in 3xTg-AD mice hippocampus at 6 and 12 months, finding increased dysregulation and 23 AD-associated ligand-receptor pairs.

This paper presents a high-quality genome assembly of the hourglass dolphin using Oxford Nanopore sequencing, achieving high contiguity and completeness for marine mammal genomics.