Author

A. Ponjavic

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Biophysics· 6 Cell Biology· 1 Immunology· 1

Signature Assays, Organisms & Methods

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Assay:imaging· 6 Approach:experimental assay· 4 Research Focus:cell type annotation· 3 Organism:human· 3 Approach:microscopy & imaging analysis· 3 Research Focus:cell signaling & trafficking· 2 Approach:statistical model· 2 Research Focus:chromatin & epigenetic regulation· 1

Frequent Co-Authors

D. Klenerman· 5 shared S. F. Lee· 4 shared S. J. Davis· 4 shared A. M. Santos· 3 shared J. McColl· 3 shared A. R. Carr· 2 shared K. O'Holleran· 2 shared L.-M. Needham· 2 shared

Activity by Year

2018-2024 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 35 mutual co-author links

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5 shared papersBiophysics

D. Klenerman

Mutual co-authors in cluster

Author profile →

8 papers

Recent·Most cited

Astigmatism-based focus stabilisation with universal objective lens compatibility, extended operating range and nanometre precision

A. Rahmani, T. Cox, A. Thamaravelil Abhimanue Achary, A. Ponjavic

This paper introduces a beam-based focus stabilisation method using astigmatism, compatible with various objective lenses and offering nanometre precision and extended operating range.

bioRxiv · Jan 15, 2024Biophysics

resPAINT: Accelerating volumetric super-resolution localisation microscopy by active control of probe emission

E. Sanders, A. R. Carr, E. Bruggeman, M. Koerbel, S. I. Benaissa, R. Donat, A. M. Santos, J. McColl, K. O'Holleran, D. Klenerman, S. J. Davis, S. F. Lee, A. Ponjavic

The paper introduces resPAINT, a method that enhances volumetric super-resolution microscopy by actively controlling probe emission, achieving up to 50-fold faster localisation rates without sacrificing contrast.

bioRxiv · Apr 14, 2022Biophysics

Single-molecule light-sheet microscopy with local nanopipette delivery

B. Li, A. Ponjavic, W.-H. Chen, L. Hopkins, C. Hughes, Y. Ye, C. E. Bryant, D. Klenerman

This paper introduces ldSPIM, a method combining light sheet microscopy with nanopipette delivery to precisely deliver single molecules to cells and image their effects in real-time.

bioRxiv · Sep 25, 2020Biophysics

Single Molecule Light Field Microscopy

R. R. Sims, S. Abdul Rehman, M. O. Lenz, A. Clark, E. W. Sanders, A. Ponjavic, L. Muresan, S. F. Lee, K. O'Holleran

This paper introduces SMLFM, a 3D single molecule localization technique with 20 nm precision, and demonstrates its application to image fixed eukaryotic cell membranes below the diffraction limit.

bioRxiv · May 20, 2020 · PreprintBiophysics

Live-cell 3D single-molecule tracking reveals how NuRD modulates enhancer dynamics

S. Basu, O. Shukron, D. Hall, P. Parruto, A. Ponjavic, D. Shah, W. Boucher, D. Lando, W. Zhang, N. Reynolds, L. H. Sober, A. Jartseva, R. Ragheb, X. Ma, J. Cramard, , E. D. Laue

This study uses live-cell 3D single-molecule tracking to show that the NuRD complex decompacts chromatin and alters enhancer dynamics by increasing their movement and reducing time in a fast-diffusing state.

bioRxiv · Apr 3, 2020 · PreprintCell Biology

vLUME: 3D Virtual Reality for Single-molecule Localization Microscopy

A. Spark, A. Kitching, D. Esteban-Ferrer, A. Handa, A. R. Carr, L.-M. Needham, A. Ponjavic, M. Da Cunha Santos, J. McColl, C. Leterrier, S. J. Davis, R. Henriques, S. F. Lee

vLUME is a VR software for visualizing 3D SMLM data, enabling segmentation and quantification of fluorescence puncta with high fidelity.

bioRxiv · Jan 20, 2020Biophysics

The costs of close contact: Visualizing the energy landscape of cell contacts at the nanoscale

A. Lippert, K. Kulenkampff, J. McColl, D. Klenerman, A. M. Santos, A. Ponjavic, S. Lee, S. J. Davis

This paper uses quantum dots to study how protein size affects diffusion and entry into cell contacts, revealing size-dependent exclusion and reduced diffusion in dense adhesion protein regions.

bioRxiv · Jul 9, 2019Biophysics

A cell-topography based mechanism for ligand discrimination by the T-cell receptor

R. A. Fernandes, K. A. Ganzinger, J. Tzou, P. Jonsson, S. F. Lee, M. Palayret, A. M. Santos, A. A. Carr, A. Ponjavic, V. T. Chang, C. Macleod, B. C. Lagerholm, A. E. Lindsay, O. Dushek, A. Tilevik, , D. Klenerman

This paper develops a model showing T-cell receptors discriminate ligands based on dwell-time in phosphatase-depleted contacts constrained by cell topography, predicting signaling potency without multi-step proof-reading.

bioRxiv · Nov 19, 2018Immunology