A. M. Santos

The kinase occupancy of T-cell coreceptors reconsidered

The paper re-evaluates kinase occupancy of T-cell coreceptors CD4 and CD8β, finding CD4-Lck interaction is stoichiometric while CD8β-Lck is substantial, with structural differences influencing binding.

resPAINT: Accelerating volumetric super-resolution localisation microscopy by active control of probe emission

The paper introduces resPAINT, a method that enhances volumetric super-resolution microscopy by actively controlling probe emission, achieving up to 50-fold faster localisation rates without sacrificing contrast.

Evidence for Deleterious Antigenic Imprinting in SARS-CoV-2 Immune Response

This study identifies cross-reactive antibodies against SARS-CoV-2 Ep9 epitope that originated from prior influenza infection, suggesting antigenic imprinting contributes to severe COVID-19 outcomes.

Aggregation and immobilisation of membrane proteins interplay with local lipid order in the plasma membrane of T cells

The paper investigates how membrane protein aggregation and immobilization affect lipid order in T cells, revealing lipid-mediated recruitment of signaling proteins like Lck.

Predicting COVID-19 Severity with a Specific Nucleocapsid Antibody plus Disease Risk Factor Score

This paper identifies an antibody epitope and a risk score to predict severe COVID-19 outcomes with high specificity.

Robust T-cell signaling triggered on soft polydimethylsiloxane-supported lipid-bilayers

This study investigates T-cell signaling on soft lipid bilayers, finding early calcium signaling is not mechanosensitive but phosphatase exclusion occurs on soft substrates.

The costs of close contact: Visualizing the energy landscape of cell contacts at the nanoscale

This paper uses quantum dots to study how protein size affects diffusion and entry into cell contacts, revealing size-dependent exclusion and reduced diffusion in dense adhesion protein regions.

A cell-topography based mechanism for ligand discrimination by the T-cell receptor

This paper develops a model showing T-cell receptors discriminate ligands based on dwell-time in phosphatase-depleted contacts constrained by cell topography, predicting signaling potency without multi-step proof-reading.

A novel role for Myosin-Va in mitochondrial fission

This paper shows myosin-Va facilitates mitochondrial fission in melanoma cells by interacting with Spire1C and localizing to fission sites, linking it to the Warburg effect.

Hydrodynamic trapping measures the interaction between membrane-associated molecules

The paper introduces hydrodynamic trapping to measure membrane protein interaction potentials and shows how protein shape, glycosylation, and flexibility affect their behavior.

Reconstitution of immune cell interactions in free-standing membranes

The paper presents an in vitro system using synthetic vesicles to study spatiotemporal aspects of immune synapse formation and signaling molecule distribution in T cells.