A. W. Purcell

A Simple and Rapid Protocol for the Isolation of Murine Bone Marrow Suitable for the Differentiation of Dendritic Cells

This paper introduces a centrifugation-based method for isolating murine bone marrow to differentiate dendritic cells, which increases cell numbers and yields more type 1 conventional dendritic cells.

Conserved allomorphs of MR1 drive specificity of MR1-restricted TCRs

The paper shows that MR1-restricted TCRs recognize an allelic variant of MR1 (MR1*04) present in healthy individuals, indicating that these TCRs are not cancer-specific and may react to both cancer and healthy cells.

Endogenous viral elements constitute a complementary source of antigens for personalized cancer vaccines

This paper identifies endogenous viral elements as a new source of antigens for personalized cancer vaccines and introduces a computational pipeline to predict and test their therapeutic potential.

The self-peptide repertoire plays a critical role in transplant tolerance induction

This study identifies immunogenic MHC I peptides involved in transplant tolerance by combining genetic engineering, immunopeptidomics, and T cell analysis.

Passage of transmissible cancers in the Tasmanian devil is due to a dominant, shared peptide motif and a limited repertoire of MHC-I allotypes

The paper identifies a shared peptide motif and limited MHC-I diversity in Tasmanian devil transmissible cancers, suggesting a mechanism for immune evasion and vaccine design.

A peptide-signal amplification strategy for the detection and validation of neoepitope presentation on cancer biopsies

The paper develops a mass spectrometry-based method to detect HLA-bound peptides from tumor biopsies, enabling the identification of neoepitopes from as few as 1000 cells or 1 mg of tissue.

Spliced peptides and cytokine driven changes in the immunopeptidome of melanoma

This study identifies spliced peptides and cytokine-driven changes in the immunopeptidome of melanoma, showing conserved immunogenic peptides across patients.

HLA-class II specificity assessed by high-density peptide microarray interactions

This study uses high-density peptide microarrays to assess HLA-class II specificity, generating large-scale peptide-MHC-II interaction data to improve prediction tools.

Benchmarking predictions of MHC class I restricted T cell epitopes

This study benchmarks T cell epitope prediction tools using a comprehensive dataset of vaccinia virus epitopes, finding that neural network-based methods trained on MHC binding and ligand elution data perform best.

Viral hijacking of the nucleolar DNA-damage response machinery: a novel mechanism to regulate host cell biology

The paper identifies a novel viral mechanism that hijacks the nucleolar DNA-damage response pathway by interacting with Treacle to inhibit rRNA biogenesis.

Tadpole-like conformations of huntingtin exon 1 with expanded polyglutamine engenders novel interactions in cells

The paper investigates the structure of Httex1 with expanded polyQ and finds that its tadpole-like conformation increases interactions in cells, potentially contributing to neurotoxicity.