M. Delarue

Vestibular modulation by stimulant derivatives in a pentameric ligand-gated ion channel

This study investigates how psychostimulant derivatives modulate the prokaryotic pentameric ligand-gated ion channel sTeLIC through a vestibular pocket, revealing a structure-function mechanism for allosteric potentiation.

Macromolecular interactions and geometrical confinement determine the 3D diffusion of ribosome-sized particles in live Escherichia coli cells

This study tracks ribosome-sized particles in E. coli to show that their 3D diffusion is influenced by macromolecular interactions and geometrical confinement, revealing sub-diffusive motion due to nucleoid and cell boundary constraints.

Discovering cryptic pocket opening and binding of a stimulant derivative in a vestibular site of the 5-HT3A receptor

The paper identifies a stimulant derivative binding in a cryptic vestibular pocket of the 5-HT3A receptor using computational and experimental methods.

Parallel on-chip micropipettes enabling quantitative multiplexed characterization of vesicle mechanics and cell aggregates rheology

This paper introduces a high-throughput method for quantitatively measuring mechanical properties of biological samples using parallel micropipettes.

Mechanical characterization of regenerating Hydra tissue spheres

This study characterizes the mechanical properties of Hydra vulgaris tissue spheres using microaspiration and simulations, revealing three distinct mechanical behaviors during regeneration.

An easy-to-use microfluidic mechano-chemostat for tissues and organisms reveals that confined growth is accompanied with increased macromolecular crowding

This paper introduces a microfluidic device that enables controlled mechanical and chemical environments for studying biological samples, showing that confined growth increases macromolecular crowding.

DNA-binding mechanism and evolution of Replication Protein A

This study characterizes the DNA-binding mechanism of RPA from Pyrococcus abyssi using crystal and cryo-EM structures, revealing conserved features and a novel archaeal-specific domain.

nucGEMs probe the biophysical properties of the nucleoplasm

The paper introduces nucGEMs, fluorescent nanoparticles that probe nucleoplasm biophysical properties, revealing differences in motility, exclusion from heterochromatin, and effects of mitotic condensation.

Macromolecular crowding limits growth under pressure

The paper shows that growth-induced pressure in confined cells increases macromolecular crowding, which hinders protein expression and reduces growth, without requiring specific molecular sensors.

Microwell array-based opto-electrochemical detections revealing co-adaptation of rheological properties and oxygen metabolism in budding yeast

The paper develops a microwell array device for dual optical and electrochemical detection of yeast cell metabolism and rheology, showing glucose affects respiration and nanoparticle diffusion.

Structural basis for the increased processivity of D-family DNA polymerases in complex with PCNA

The paper determines the cryo-EM structure of the PolD-PCNA complex from Pyrococcus abyssi, revealing the molecular basis for increased processivity through PCNA interaction.

Mechanical-control of cell proliferation increases resistance to chemotherapeutic agents

The paper shows that compressive stress in pancreatic cancer spheroids reduces chemotherapeutic efficacy by decreasing cell proliferation.

mTORC1 controls phase-separation and the biophysical properties of the cytoplasm by tuning crowding

The paper investigates how mTORC1 regulates macromolecular crowding and biophysical properties of the cytoplasm by using GEMs to measure diffusion and phase-separation changes.

The SCWISh network is essential for survival under mechanical pressure.

The paper identifies a mechanosensitive pathway, SCWISh, essential for yeast survival under mechanical pressure by integrating signals from the SMuSh and CWI pathways.

A simple molecular mechanism explains multiple patterns of cell-size regulation

The paper proposes a molecular mechanism that explains both adder and sizer behaviors in cell-size regulation using yeast data.