J. T. Groves

Differential Roles of Kinetic On- and Off-Rates in T-Cell Receptor Signal Integration Revealed with a Modified Fab'-DNA Ligand

This paper introduces a modified Fab'-DNA ligand to study TCR signal integration by controlling kinetic on- and off-rates, revealing how dwell time affects TCR signaling and LAT condensation.

Proofreading and single-molecule sensitivity in T-cellreceptor signaling by condensate nucleation

This paper shows that T-cell receptor signaling uses condensate nucleation to selectively detect single foreign peptides by amplifying weak signals and setting spatial-temporal thresholds.

Conditional Requirement for Dimerization of the Membrane-Binding Module of BTK

This study investigates whether BTK dimerization via its PH-TH module is required for signaling in B and T cells, finding it is not necessary in B cells but essential in T cells.

Utilization of 5-deoxy-nucleosides as Growth Substrates by Extraintestinal Pathogenic E. coli via the Dihydroxyacetone Phosphate Shunt

This paper shows that in Extraintestinal Pathogenic E. coli, the DHAP shunt assimilates 5-deoxy-nucleosides as growth substrates rather than recycling sulfur or supporting methionine synthesis.

Bimodality in Ras signaling originates from processivity of the Ras activator SOS without classic kinetic bistability

The paper shows that Ras signaling bimodality arises from SOS processivity, not kinetic bistability, and is controlled by scaffold protein condensation.

A membrane-associated light harvesting model is enabled by functionalized assemblies of gene-doubled TMV proteins

This paper develops a synthetic light harvesting model using modified TMV proteins attached to lipid bilayers, enabling dynamic energy transfer studies.

Allosteric Inhibition of the T Cell Receptor by a Designed Membrane Ligand

This paper tests the allosteric model of TCR activation by designing a membrane ligand that inhibits TCR signaling, supporting the model and suggesting therapeutic applications.

Discrete LAT condensates encode antigen information from single pMHC:TCR binding events

The paper shows that LAT condensates form in response to pMHC:TCR binding events, with condensation probability linked to binding dwell time and a mutation affecting this process.

A two-component protein condensate of EGFR and Grb2 regulates Ras activation at the membrane

This study shows that EGFR and Grb2 form a condensate that regulates Ras activation through phosphorylation and dimerization.

Kinetic frustration by limited bond availability controls the LAT protein condensation phase transition on membranes

This study shows that LAT protein condensation on membranes is kinetically frustrated by limited bond availability, leading to dynamically arrested extended states rather than equilibrium compact domains.

Membrane-mediated dimerization potentiates PIP5K lipid kinase activity

This study shows that PIP5K dimerization enhances its catalytic efficiency and stochastic behavior through membrane-mediated interactions with PI(4,5)P2.

Height, but not binding epitope, affects the potency of synthetic TCR agonists

This paper investigates how the geometry of synthetic TCR agonists affects T cell activation, finding that ligand height, not epitope, determines potency.

Raf promotes dimerization of the Ras G-domain with increased allosteric connections

This study shows Raf-RBD induces Ras dimerization and identifies allosteric connections between Raf-RBD residues that contribute to signaling complex formation.

Probe the effect of clustering on EphA2 receptor signaling efficiency by subcellular control of ligand-receptor mobility

The paper investigates how EphA2 receptor clustering affects signaling efficiency by using substrates with controlled ligand mobility, finding that clustering increases phosphorylation and binding dwell time for signaling modules.

Coupling between lipid miscibility and phosphotyrosine driven protein condensation at the membrane

The paper shows that LAT protein condensation on membrane surfaces drives lipid phase separation, which in turn localizes K-Ras into the condensate, controlled by tyrosine phosphorylation.

Flexible linkers in CaMKII control the balance between activating and inhibitory autophosphorylation

This study shows that differences in kinase-hub linker lengths between CaMKII and CaMKII-β isoforms affect their autophosphorylation balance, with CaMKII favoring activation and CaMKII-β favoring inhibition.

Single-cell analysis of EphA clustering phenotypes to probe cancer cell heterogeneity

The paper develops a single-cell assay to analyze EphA receptor clustering in cancer cells, revealing its persistence through division and association with migration and drug resistance.

Force dependence of filopodia adhesion: involvement of myosin II and formins

This study investigates how forces affect filopodia adhesion, showing that myosin IIA and formins are essential for force-dependent adhesion and substrate preference.

Switch-like activation of Bruton’s tyrosine kinase by membrane-mediated dimerization

The paper identifies a switch-like PIP3 sensing mechanism in Btk's PH-TH module that enhances sensitivity through dimerization and multiple PIP3 binding.

mTORC1 controls phase-separation and the biophysical properties of the cytoplasm by tuning crowding

The paper investigates how mTORC1 regulates macromolecular crowding and biophysical properties of the cytoplasm by using GEMs to measure diffusion and phase-separation changes.