David R. Siemieniak

Mapping the Mutational Landscape for Streptokinase Binding to Plasminogen

This study maps the mutational landscape of streptokinase (SK) from Group C Streptococcus to identify amino acid substitutions affecting its binding to human plasminogen.

A Sensitized ENU Mutagenesis Screen for Thrombosis Modifiers Identifies Suppressor Variants in Non-mutagenized Parental Generations Due to Antithrombotic Selective Pressures

The paper identifies four spontaneous mutations that confer survival in a thrombosis model by reducing platelet aggregation and altering gene expression.

Thermodynamics and selection of the plasminogen activator inhibitor-1 latency transition

This study uses deep mutational scanning to analyze how PAI-1 variants affect its latency transition and uPA inhibition, showing that thermodynamic stability and evolution shape its function.

High-throughput amino acid-level characterization of the interactions of plasminogen activator inhibitor-1 with variably divergent proteases

This study uses deep mutational scanning to analyze PAI-1's inhibitory interactions with three serine proteases, revealing sequence determinants and evolutionary conservation of specificity.

Synergy of LPS and Shiga-toxin exposure on the RNA expression program of cultured human umbilical vein endothelial cells.

This study examines how LPS and Shiga toxin exposure affect gene expression in endothelial cells, finding synergistic effects at 24 hours.

The in vivo endothelial cell translatome is highly heterogeneous across vascular beds

The paper uses EC-TRAP to analyze endothelial cell translatomes across vascular beds, revealing heterogeneity in gene expression and improved detection of low-abundance transcripts.

Whole exome sequencing of ENU-induced thrombosis modifier mutations in the mouse

The paper identifies a de novo mutation in Plcb4 that suppresses thrombosis in mice with Factor V Leiden and tissue factor pathway inhibitor mutations.

A sensitized mutagenesis screen in Factor V Leiden mice identifies novel thrombosis suppressor loci

The paper identifies novel thrombosis suppressor loci using a sensitized mutagenesis screen in Factor V Leiden mice, finding F8 and the Tfpi/F3 axis, as well as Actr2, play key roles in thrombosis regulation.