David Ginsburg

Mapping the Mutational Landscape for Streptokinase Binding to Plasminogen

This study maps the mutational landscape of streptokinase (SK) from Group C Streptococcus to identify amino acid substitutions affecting its binding to human plasminogen.

Pathogenic MYBPC3 missense variants alter protein-protein interactions within the sarcomere

This study identifies pathogenic MYBPC3 missense variants in HCM patients and shows they disrupt protein interactions and alter proximity to thin-filament proteins.

Thermodynamics and selection of the plasminogen activator inhibitor-1 latency transition

This study uses deep mutational scanning to analyze how PAI-1 variants affect its latency transition and uPA inhibition, showing that thermodynamic stability and evolution shape its function.

Tandem duplication of serpin genes yields functional variation and snake venom inhibitors.

The paper studies gene duplication in SERPINA genes across vertebrates and shows that duplicated paralogs in rodents can inhibit snake venom proteases, suggesting adaptive functional variation.

Species-specific, multifaceted venom resistance in Monodelphis domestica reveals novel physiological behavior of von Willebrand Factor under flow.

This study investigates venom resistance in Monodelphis domestica, finding it resists venom-induced coagulation disruption and has unique von Willebrand Factor behavior under flow.

High-throughput amino acid-level characterization of the interactions of plasminogen activator inhibitor-1 with variably divergent proteases

This study uses deep mutational scanning to analyze PAI-1's inhibitory interactions with three serine proteases, revealing sequence determinants and evolutionary conservation of specificity.

Synergy of LPS and Shiga-toxin exposure on the RNA expression program of cultured human umbilical vein endothelial cells.

This study examines how LPS and Shiga toxin exposure affect gene expression in endothelial cells, finding synergistic effects at 24 hours.

Functional overlap between the mammalian SAR1A and SAR1B paralogs in vivo

The paper shows that SAR1A and SAR1B paralogs have overlapping functions in mice, with SAR1A compensating for SAR1B deficiency.

Regulation of cellular LDL uptake by PROX1 and CHD7

This study identifies PROX1 and CHD7 as regulators of LDL uptake, with PROX1 directly enhancing LDLR expression and CHD7 acting through an LDLR-independent mechanism.

Genome-scale CRISPR screening for modifiers of cellular LDL uptake

The paper uses genome-scale CRISPR screening to identify genetic modifiers of LDL uptake in hepatocytes, finding several new genes involved in this process.

The in vivo endothelial cell translatome is highly heterogeneous across vascular beds

The paper uses EC-TRAP to analyze endothelial cell translatomes across vascular beds, revealing heterogeneity in gene expression and improved detection of low-abundance transcripts.

Whole exome sequencing of ENU-induced thrombosis modifier mutations in the mouse

The paper identifies a de novo mutation in Plcb4 that suppresses thrombosis in mice with Factor V Leiden and tissue factor pathway inhibitor mutations.

Murine SEC24D Can Substitute Functionally for SEC24C in vivo

This study shows that SEC24D can functionally substitute for SEC24C in mice, as Sec24cc-d/c-d pups survive to term despite embryonic lethality in Sec24c null mice.

A sensitized mutagenesis screen in Factor V Leiden mice identifies novel thrombosis suppressor loci

The paper identifies novel thrombosis suppressor loci using a sensitized mutagenesis screen in Factor V Leiden mice, finding F8 and the Tfpi/F3 axis, as well as Actr2, play key roles in thrombosis regulation.