G. R. Hammond

PILS-Nir1 is a novel phosphatidic acid biosensor that reveals mechanisms of lipid production

The paper develops PILS-Nir1, a phosphatidic acid biosensor, and shows it detects PA dynamics in live cells with higher sensitivity than existing tools.

MTMR regulates KRAS function by controlling plasma membrane levels of phospholipids

The paper identifies MTMR proteins as regulators of KRAS function by controlling plasma membrane phospholipid levels, specifically PI4P and PtdSer.

OSBP is a major determinant of Golgi phosphatidylinositol 4-phosphate homeostasis

The paper investigates OSBP's role in maintaining Golgi PI4P homeostasis through genetic manipulations and finds OSBP regulates PI4P levels at ER:Golgi contact sites.

Orthogonal targeting of SAC1 to mitochondria implicates ORP2 as a major player in PM PI4P turnover

The paper investigates PM PI4P turnover by targeting SAC1 to mitochondria, finding that ORP2 significantly affects PM PI4P levels independently of contact sites.

Missense mutations in the calcium-activated chloride channel TMEM16A promote tumor growth by activating oncogenic signaling in Human Cancer.

This paper investigates TMEM16A missense mutations in cancer, finding they promote tumor growth through altered signaling, independent of chloride flux.

Depletion of Plasma Membrane PI4P by ORP5 Requires Hydrolysis by SAC1 in Acceptor Membranes

The authors retract their paper due to an incorrect conclusion about ORP5's role in PI4P depletion, finding SAC1 targeting alone suffices.

A Novel Homeostatic Mechanism Tunes PI(4,5)P2-dependent Signaling at the Plasma Membrane

The paper identifies PIP4K as a sensor and negative regulator of PI(4,5)P2 synthesis, revealing a homeostatic mechanism that tunes membrane functions.

PtdIns(3,4)P2, Lamellipodin, and VASP coordinate cytoskeletal remodeling during phagocytic cup formation in macrophages.

Free diffusion of PI(4,5)P2 in the plasma membrane in the presence of high density effector protein complexes

The paper measures PI(4,5)P2 diffusion in the plasma membrane and finds that it diffuses rapidly even in regions with high effector protein density, suggesting free diffusion rather than spatial segregation.

Dynamic Turnover of PI4P and PI(4,5)P2 under Hypoxia Controls Electrostatic Plasma Membrane Targeting

The paper shows hypoxia causes reversible depletion of PM PI4P and PIP2, disrupting electrostatic targeting of polybasic proteins, and identifies PI4KIII and its regulators as essential for maintaining their homeostasis.

Phosphatidylinositol 3-kinase-independent synthesis of PtdIns(3,4)P2 by a phosphotransferase

The paper shows that Salmonella and Shigella effectors generate PtdIns(3,4)P2 independently of phosphoinositide 3-kinases using a phosphotransferase mechanism.

Probing the Subcellular Distribution of Phosphatidylinositol Reveals a Surprising Lack at the Plasma Membrane

The study maps the subcellular distribution of phosphatidylinositol (PI) and finds it is largely absent from the plasma membrane, where it is mainly present as PI4P and PI(4,5)P2.

A High Avidity Biosensor Reveals PI(3,4)P2 is Predominantly a Class I PI3K Signaling Product

The paper develops a high-avidity biosensor for PI(3,4)P2, revealing it is mainly produced by class I PI3K and hydrolyzed by PTEN.