David Horn

This study uses a genetic screen in Trypanosoma brucei to identify novel kDNA maintenance factors, validates one as a novel replication factor, and provides a prioritized list of candidates.

The paper uses MOT-library profiling to assess all possible mutations at a drug binding site and predicts resistance mechanisms through computational modeling.

This paper identifies ZNK1 as a zinc-sensing RNA-binding protein in trypanosomes that degrades ZIP3 mRNA in response to zinc availability.

This study identifies temperature-sensitive mRNA structures and shows that argonaute-dependent transcriptome remodelling mediates thermo-sensing in trypanosomes.

This paper identifies mutations in the gamma subunit of the mitochondrial ATP synthase in Trypanosoma brucei that lead to kinetoplast loss and drug resistance.

The paper identifies mutations in trypanosome CPSF3 that confer resistance to acoziborole, a drug targeting RNA processing, and explains the molecular mechanism of resistance through computational modeling.

The paper shows that VSG transcripts play a non-coding role in allelic exclusion in trypanosomes by participating in allelic competition.

The paper shows that specific histone H4 lysine mutations disrupt polycistronic gene expression in trypanosomes by altering promoter-associated chromatin structure.

This study identifies CPSF3 as a target of benzoxaboroles in trypanosomatids, showing that BoBs induce sumoylation-dependent degradation of CPSF complex members, leading to reduced mRNA levels.

The paper identifies a novel mechanism in trypanosomes where inter-chromosomal interactions between an antigen coding gene and an mRNA splicing locus ensure monogenic antigen expression.

The paper performs a genome-wide RNAi screen in Trypanosoma brucei to identify cell cycle regulators, identifying hundreds of proteins involved in different stages of the cell cycle and providing functional genomic insights.

The paper explains a VSG hierarchy in Trypanosoma brucei infections through a growth-rate mechanism linked to VSG length, showing shorter VSGs trigger immune responses while longer VSGs persist due to slower growth.