Florian Fröhlich

Systematic Analysis of GARP- and EARP-Mediated Trafficking Pathways Using Lysosomal Proteomics

This study uses lysosomal proteomics and knockdowns to analyze the cargo spectra of GARP and EARP complexes, revealing their distinct roles in protein trafficking and their links to neurodegenerative disorders.

Cholesterol transport cycle of human ABCA2

This study reports cryo-EM structures of human ABCA2, revealing its cholesterol transport mechanism and novel conformational changes.

Ire1 membrane stress responses support cell growth upon disruptions in inter-organelle contacts

This study shows that Ire1-dependent Membrane Stress Responses (MSR) help yeast cells grow when inter-organelle contacts are disrupted by compensating for lipid metabolism defects and modulating Ca2+ signaling.

VPS13C/PARK23 initiates lipid transfer and membrane remodeling for efficient lysosomal repair

This study identifies VPS13C/PARK23 as a key protein involved in lysosomal repair by facilitating lipid transfer and membrane remodeling in response to damage.

ESCRT proteins organise stress-induced organelle contact between endoplasmic reticulum and Golgi

The paper shows ESCRT proteins, specifically Snf7 and Bro1, organize stress-induced ER-Golgi contacts in yeast by forming tubular ER subdomains and recruiting tethering proteins.

Lipid Droplet Targeting Drives Substrate Access during Very Long Chain Fatty Acid Activation by Fat1

This study shows lipid droplets are the site of VLCFA activation by Fat1, with Fat1 targeting LDs via an amphipathic helix and using a hydrophobic cavity to access substrates.

The Myo2 adaptor Ldm1 and its receptor Ldo16 mediate actin-dependent lipid droplet motility

The paper identifies Ldm1 and Ldo16 as actin-dependent lipid droplet motility mediators in yeast, showing their roles in LD movement and mitochondrial transport.

Steric Repulsion Counteracts ER-to-Lipid Droplet Protein Movement

The paper identifies steric repulsion as a mechanism regulating ER-to-LD protein movement, showing high-affinity proteins exclude lower-affinity ones during adipocyte differentiation.

Uptake of exogenous serine is important to maintain sphingolipid homeostasis in Saccharomyces cerevisiae

This study shows that serine uptake via Gnp1 is critical for maintaining sphingolipid homeostasis in yeast by linking serine import to sphingolipid biosynthesis.