Manthan N. Patel

Targeting DNA-LNPs to Endothelial Cells Improves Expression Magnitude, Duration, and Specificity

This study shows that targeting DNA-LNPs to endothelial cells enhances transgene expression magnitude, duration, and specificity in target organs.

Lipid Nanoparticle-Associated Inflammation is Triggered by Sensing of Endosomal Damage: Engineering Endosomal Escape without Side Effects

This paper identifies that lipid nanoparticle-induced inflammation arises from endosomal membrane damage and proposes strategies to mitigate it through galectin inhibition or ESCRT-recruiting lipids.

Enabling non-viral DNA delivery using lipid nanoparticles co-loaded with endogenous anti-inflammatory lipids

This study develops lipid nanoparticles co-loaded with anti-inflammatory lipids to reduce inflammation from DNA delivery, enabling prolonged transgene expression.

Multi-stage-mixing to control the supramolecular structure of lipid nanoparticles, thereby creating a core-then-shell arrangement that improves performance by orders of magnitude

This paper introduces multi-stage-mixing (MSM) to control lipid nanoparticle (LNP) supramolecular structure, creating a core-then-shell arrangement that significantly improves mRNA and DNA-LNP performance.

Marginated neutrophils in the lungs effectively compete for nanoparticles targeted to the endothelium, serving as a part of the reticuloendothelial system

The paper shows that lung marginated neutrophils effectively uptake endothelial-targeted nanoparticles, suggesting they are part of the reticuloendothelial system.