Michael Guy Poirier

This study shows how the Cre recombinase uses a sequestered conformation to enhance DNA scanning efficiency and target specificity by modulating its binding affinity and diffusion behavior.

This study investigates how linker histone H1.0 binds to nucleosomes and DNA, revealing multiple pathways facilitated by histone chaperones like Nap1.

This study investigates how EWSR1::FLI1 binds to nucleosomes and DNA motifs, revealing its pioneer factor properties and preference for GGAA repeats.

The paper shows that cohesin facilitates nucleosome invasion by transcription factors, even in the absence of chromatin remodeling or replication.

This paper introduces Free Energy Spectroscopy (FES) to characterize chromatin compaction by analyzing end-to-end distance and nucleosome stacking interactions, revealing three mechanisms by which linker histones reshape compaction energetics.

This paper introduces a method to isolate ssDNA from dsDNA sources for DNA origami assembly, demonstrating its effectiveness across various dsDNA types and lengths.

The paper characterizes native nucleosome-positioning elements in yeast and shows they can be used to study nucleosome repositioning by transcription factors and chromatin remodelers.

This study shows that H2B variants alter chromatin structure and oncogenic gene expression, suggesting they may be early cancer biomarkers and therapeutic targets.

This paper introduces methods to recycle DNA components in DNA origami manufacturing, demonstrating the reprogramming of nanostructures and recovery of staple strands to create sustainable, reusable devices.

This study shows that ATAC and SAGA HAT modules enhance transcription factor binding to nucleosomes without requiring histone acetylation.

This study uses single-molecule imaging to show that Gal4 dwell time determines transcriptional burst size in yeast, with nucleosomes reducing dwell time and affecting transcriptional bursting kinetics.

This study investigates how budding yeast pioneer transcription factors bind and dissociate from nucleosomal DNA, showing they compensate for reduced binding by increasing dwell time, similar to human pioneer factors.