Thomas Harry Sharp

Cell-Surface RNA Forms Ternary Complex with RNA-Binding Proteins and Heparan Sulfate to Recruit Immune Receptors

The paper identifies heparan sulfate (HS) as a key factor for cell-surface RNA (csRNA) presentation and reveals a ternary complex of HS, RNA, and RNA-binding proteins that recruits immune receptors.

Scaling data analyses in cellular cryoET using comprehensive segmentation

The paper presents a comprehensive segmentation strategy for cryoET data using convolutional neural networks, enabling efficient analysis of large datasets and improving particle picking and template matching.

Structural basis for surface activation of the classical complement cascade by the short pentraxin C-reactive protein

This paper presents a cryo-ET structure of CRP bound to C1 complex and PC ligands, revealing how CRP activates complement and identifies residues for therapeutic targeting.

DNA nanostructure-templated antibody complexes provide insights into the geometric requirements of human complement cascade activation

This study uses DNA nanostructures to template IgG antibodies and investigate how IgG valency affects complement activation, revealing that antigen number correlates with complement cleavage and terminal pathway activation.

Correlating Protein Aggregate Structure with Cellular Function in Differentiated Muscle Cells: Discriminating Pathogenic from Non-Pathogenic Forms

This study correlates the structure of pathogenic and non-pathogenic PABPN1 protein aggregates with cellular function in differentiated muscle cells using multimodal imaging.

Ais: streamlining segmentation of cryo-electron tomography datasets

Ais provides a user-friendly, high-performance tool for cryo-electron tomography segmentation, accelerating particle picking and cellular architecture analysis.

Design and synthesis of DNA origami nanostructures to control TNF receptor activation

The paper designs and characterizes DNA origami nanostructures decorated with SC-TRAIL complexes to enhance TNFR activation and cell killing in Jurkat cells.

Building a super-resolution fluorescence cryomicroscope

The paper describes the design of a super-resolution fluorescence cryomicroscope for single molecule localization microscopy of cryosamples.

Imaging intracellular components in situ using super-resolution cryo-correlative light and electron microscopy

This paper outlines a workflow for SRcryoCLEM, demonstrating its application using Vimentin and showing how it enables targeted imaging of intracellular components in situ.

Super-resolution fluorescence imaging of cryosamples does not limit achievable resolution in cryoEM

The paper investigates whether super-resolution fluorescence imaging before cryoEM affects resolution, finding no detrimental effect.

Selecting optimal support grids for super-resolution cryogenic correlated light and electron microscopy

The paper investigates how support grid design in cryo-TEM affects fluorescence microscopy illumination and demonstrates improvements in super-resolution image quality through optimal grid selection.