Andrey S. Klymchenko

Cerebral microthrombi promote focal Cav-1-dependent blood-brain barrier impairment, triggering neuroinflammation and neuronal damage after traumatic brain injury

This study identifies cerebral microthrombi as focal sites of Cav-1-dependent BBB dysfunction after TBI, linking microvascular thrombosis to neuronal damage through transcytosis.

Covalent Solvatochromic Membrane Probe for Sensing Lipid Surrounding of Proteins

This paper introduces a new fluorescent probe, MemGraft-NR, for sensing lipid organization around proteins, showing higher sensitivity to cholesterol and revealing new insights into protein-lipid interfaces in live cells and endosomes.

Rhodamine-derived ratiometric fluorescent molecular rotor for mitochondrial viscosity sensing

This paper introduces a new ratiometric fluorescent molecular rotor for mitochondrial viscosity sensing, showing it can quantify inner mitochondrial membrane viscosity and reveal heterogeneity under oxidative stress.

Endosomal membrane state governs trafficking and cell migration under TFEB control

This study shows TFEB regulates endosomal membrane fluidity and cargo trafficking, affecting cell migration and adhesion in bladder cancer.

Fluorescence based microviscosity mapping in membraneless organelles

This study uses BODIPY-based molecular rotors and FLIM to measure microviscosity in membraneless organelles, revealing differences in viscosity across sub-compartments and changes under perturbation.

Prodan-based solvatochromic probes for polarity imaging of organelles

The paper introduces Prodan-based solvatochromic probes for imaging organelle polarity, showing organelle-specific polarity changes under starvation.

Peptide-functionalized fluorescent polymeric nanoparticles: polysarcosine length defines stealth properties

This study shows that polysarcosine length affects nanoparticle stealth properties, enabling specific protein targeting with minimal non-specific interactions.

Fluorescent anionic cyanine plasma membrane probes for live cell and in vivo imaging

The paper introduces anionic cyanine-based plasma membrane probes for live cell and in vivo imaging, showing improved staining and visualization of neuronal structures in mice.

Lipid-directed covalent fluorescent labeling of plasma membranes for long-term imaging, barcoding and manipulation of cells

This paper introduces MemGraft, a covalent fluorescent labeling method for plasma membranes that enables long-term imaging, barcoding, and manipulation of cells without probe exchange.

Order disorder phase transitions in the plasma membrane of HeLa cells measured by fluorescent analysis of solvatochromic probes

This study measures order-disorder phase transitions in HeLa cell plasma membranes using fluorescent solvatochromic probes, finding reversible transitions influenced by cholesterol and temperature.

A Cell-Permeable Fluorescent Probe Reveals Temporally Diverse PI(4,5)P2 Dynamics Evoked by Distinct GPCR Agonists in Neurons

This paper develops a fluorescent probe to track PI(4,5)P2 dynamics in neurons, revealing that hallucinogenic ligands at the 5-HT2A receptor cause slower initial PI(4,5)P2 depletion compared to non-hallucinogenic ligands.

Cooperative effects in DNA-functionalized polymeric nanoparticles

This study investigates how dense DNA packing on polymeric nanoparticles enhances duplex stability and hybridization specificity for nucleic acid sensing.

Functionalization of lipid nanoemulsions with humanized antibodies using plug-and-play cholesterol anchor for targeting cancer cells

This paper develops a method to functionalize lipid nanoemulsions with antibodies for targeted cancer therapy using a cholesterol anchor and biotin-neutravidin coupling.

Nanocarrier Drug Release and Blood-Brain Barrier Penetration at Post-Stroke Microthrombi Monitored by Real-Time Förster Resonance Energy Transfer-Based Detection System (FedEcs)

This paper develops a real-time FRET-based system to monitor nanocarrier behavior and drug release at post-stroke microthrombi in vivo.

ATUM-Tomo: A multi-scale approach to cellular ultrastructure by combined volume scanning electron microscopy and electron tomography

The paper introduces ATUM-Tomo, a hybrid method combining SEM and electron tomography for multi-scale ultrastructural analysis of cellular structures.

Molecular Tuning of Styryl Dyes Leads to Versatile and Efficient Plasma Membrane Probes for Cell and Tissue Imaging

This paper develops new styryl dyes with improved photophysical properties for plasma membrane imaging in mammalian and plant cells.

MemBright: a Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience

MemBright is a family of fluorescent membrane probes that enable high-contrast, selective plasma membrane staining for advanced cellular imaging and neuroscience applications.