Thomas Misgeld

Early demyelination by off-target complement injury in a mouse model of neuromyelitis optica

This study shows that in a mouse model of NMOSD, demyelination occurs due to off-target complement injury to oligodendrocytes, not directly from astrocyte loss.

Local demyelination rekindles oligodendrocyte precursor dynamics and remyelination in old age

This study shows that OPCs in aging retain remyelination potential, which can be reactivated by demyelination, improving myelin repair.

Selective Cortical Myelination Reflects Axon-Driven Progression of the BCAS1-positive Oligodendrocyte Lineage

The paper investigates the mechanisms of selective cortical myelination, finding that early myelination is axon-driven and influenced by interactions with specific neuronal partners.

A reproducible human brain tissue model to study physiological and disease-associated microglia phenotypes

This paper develops a 3D cortical brain tissue model with mature microglia that recapitulates Alzheimer's disease hallmarks and enables study of microglia phenotypes.

Mitochondrial hitch-hiking of Mapt mRNA maintains Tau levels in axons

The paper identifies mitochondrial transport of MAPT mRNA via SYNJ2a/SYNJ2BP complex and shows its role in maintaining Tau levels and axonal growth in glutamatergic neurons.

Neuronal compartmentalization results in impoverished axonal mitochondria

The paper uses proteomics to compare mitochondrial diversity in somatodendritic and axonal compartments across neurons, finding axonal mitochondria are 'impoverished' with reduced mtDNA expression and impaired oxidative phosphorylation.

Polyglutamylation of microtubules drives neuronal remodeling

The paper shows that polyglutamylation of microtubules regulates neuronal remodeling by marking them for severing, affecting synapse and axon pruning in both peripheral and central circuits.

Axon-specific microtubule regulation drives asymmetric regeneration of sensory neuron axons

This study identifies an axon-specific microtubule-associated protein signature that drives asymmetric regeneration in sensory neuron axons, using an in vitro model of DRG neurons.

A retrograde transit filter mediated by optineurin controls mitostasis in distal axons

The paper identifies a retrograde transit filter in distal axons mediated by optineurin that regulates mitochondrial homeostasis, independent of PINK1 and parkin.

ER-associated biogenesis of PINK1 preprotein for neuronal mitophagy

The paper shows that PINK1 precursor translation in neurons requires ER and endolysosome interaction, with DNAJB6 facilitating its transport to mitochondria for mitophagy.

Astrocytes adopt a progenitor-like migratory strategy for regeneration in adult brain

The paper shows that mature astrocytes can transform into progenitor-like cells to regenerate astrocytic networks after injury in mice.

Imaging membrane damage in ferroptosis and necrosis by wash-free fluorogenic chemical probes

The paper develops fluorogenic chemical probes that detect membrane damage without washing steps, enabling in vivo imaging of cell death mechanisms like ferroptosis and necrosis.

Nanocarrier Drug Release and Blood-Brain Barrier Penetration at Post-Stroke Microthrombi Monitored by Real-Time Förster Resonance Energy Transfer-Based Detection System (FedEcs)

This paper develops a real-time FRET-based system to monitor nanocarrier behavior and drug release at post-stroke microthrombi in vivo.

ATUM-Tomo: A multi-scale approach to cellular ultrastructure by combined volume scanning electron microscopy and electron tomography

The paper introduces ATUM-Tomo, a hybrid method combining SEM and electron tomography for multi-scale ultrastructural analysis of cellular structures.

Multimodally trackable and clinically translatable platform for modelling human demyelinating brain diseases by temporally dispersed chemically induced lesions in thepig brain

This paper develops a large animal model for demyelinating brain diseases using minipigs, demonstrating reversible and multimodally trackable lesions that mimic human pathology.

Targeting the TCA cycle can ameliorate widespread axonal energy deficiency in neuroinflammatory lesions

The paper shows that TCA cycle dysfunction contributes to axonal energy deficiency in neuroinflammatory lesions and that restoring TCA enzymes can alleviate this deficit.

Transthyretin promotes axon growth via regulation of microtubule dynamics and tubulin acetylation

This study shows that Transthyretin (TTR) promotes axon growth by regulating microtubule dynamics and tubulin acetylation, with restoration of acetylated tubulin rescuing defects in TTR knockout mice.

Multiscale ATUM-FIB microscopy enables targeted ultrastructural analysis at isotropic resolution

The paper introduces ATUM-FIB, a hybrid microscopy technique that combines serial sectioning with FIB-SEM to enable targeted ultrastructural analysis at isotropic resolution.

CRMP2 mediates Sema3F-dependent axon pruning and dendritic spine remodeling

This study shows CRMP2 is essential for Sema3F-dependent axon pruning and dendritic spine remodeling, linking its dysfunction to ASD-like behaviors in mice.

Precision in a sea of profusion: Myelin replacement triggered by single-cell cortical demyelination

The paper shows that single oligodendrocyte loss in mouse cortex triggers precise remyelination and cell replacement, preserving original myelin patterns while creating new internodes on unmyelinated axons.

Localized calcium accumulations prime synapses for phagocyte removal in cortical neuroinflammation

The paper shows that calcium accumulations in dendritic spines precede their removal by phagocytes in cortical inflammation, and blocking phagocyte activation prevents synapse loss.

Iron-sequestering nanocompartments as multiplexed Electron Microscopy gene reporters

The paper introduces a genetic iron-biomineralizing nanocompartment for multiplexed EM gene reporter imaging in mammalian cells.