Mark D. Szczelkun

The paper shows that diversity in restriction-modification systems is maintained through trade-offs between defense strength and horizontal transferability, and provides collective protection against phages.

The paper defines the mechanism of the MANTIS defence system in Pseudomonas aeruginosa, showing how dual ATPases couple self-modification to non-self restriction through adenine hemimethylation and cleavage.

The paper analyzes defence system interactions in Pseudomonas aeruginosa genomes to understand their role in shaping accessory genome dynamics and ecological patterns.

This study investigates how the Staphylococcus aureus SauUSI restriction enzyme recognizes and cleaves methylated DNA through ATP-driven translocation and loop formation.

The paper presents structures of a Lamassu defence system from Salmonella enterica, revealing how LmuA nuclease effector is activated by oligomerization and DNA cleavage.

The paper investigates eco-evolutionary feedbacks between restriction-modification systems and antimicrobial resistance gene acquisition using modeling and genome analysis.

The paper examines how Restriction-Modification systems affect plasmid conjugation in E. coli, finding that plasmids often encode anti-RM strategies, reducing the effectiveness of RM systems as a barrier.

ENDO-Pore maps DNA cleavage events using nanopore sequencing, with CSI identifying cleavage sites, and it was benchmarked with restriction endonucleases and CRISPR Cas12a.

This study evaluates the effectiveness of different CRISPR effectors for targeting mitochondria, finding that LbCas12a shows better targeting and tolerance than Cas9 variants.