Vincenzo Costanzo

Mapping the genetic landscape of the DNA damage response with Cas12a-based combinatorial knockout screens

This study uses Cas12a-based knockout screens to identify genetic interactions in the DNA damage response, revealing synthetic lethal and buffering interactions with therapeutic implications.

Quantitative AI-based DNA fiber workflow to study replication stress

The paper introduces a quantitative AI-based DNA fiber workflow to analyze replication stress, demonstrating its efficiency and robustness through independent experimental datasets.

HMCES corrupts replication fork stability during base excision repair in homologous recombination deficient cells

The paper shows HMCES causes replication fork instability in HRD cells during BER of 5hmdC-derived lesions, leading to chromosomal damage and cell death.

The cohesin ring uses its hinge to organize DNA using non-topological as well as topological mechanisms

The paper investigates how cohesin organizes DNA through both topological and non-topological mechanisms, showing that hinge domain mutations affect DNA entrapment and loading.