Z. Hong Zhou

CryoID2 uses deep learning and sequence analysis to identify proteins and RNA from cryoEM maps, achieving high accuracy in molecular identification.

This study characterizes the structural dynamics of recombinant vaults, showing that cargo loading stabilizes their conformation for high-payload cytokine delivery in cancer therapy.

This study uses 4D-STEM to visualize nanoscale dynamics in protein microcrystals, revealing continuous rearrangements and radiolytic damage propagation.

This paper introduces spIsoNet, a deep-learning method to correct preferred-orientation bias in cryoEM reconstructions, demonstrating its effectiveness on influenza HA trimer datasets.

IsoNet2 reconstructs high-resolution 3D cellular structures from cryogenic electron tomography without averaging.

This study identifies IMC1 as a critical component of the Toxoplasma gondii inner membrane complex, showing its role in morphology, replication, and infectivity through expression timing, knockout, and interaction analyses.

This study identifies RBM5's role in regulating splicing by activating DHX15 and blocking spliceosome progression, with implications for cancer-related splicing dysregulation.

The paper reports the cryoEM structure of FPM13, a novel periplasmic metalloprotein from Francisella, and shows its role in metal binding and potential detoxification.

The paper describes a protein fold switch in a bactericidal nanomachine that enables membrane puncture and bactericidal activity.

This study shows that clathrins are essential for proper cytostome formation in Plasmodium falciparum, which is critical for hemoglobin uptake and digestion.

CryoDataBot automates the creation of high-quality cryoEM datasets for AI-driven structural biology, improving training efficiency and enabling flexible dataset customization.

This study identifies a new D-capsid type in herpesvirus assembly, revealing structural dynamics of DNA translocation and clarifying the origins of empty and DNA-filled capsids.

This study identifies and characterizes the RibD-enolase complex in Francisella novicida, revealing structural features and catalytic independence, with implications for antibiotic development.

This study uses cryoEM to reveal the structure of the central apparatus in Trypanosoma brucei flagella, identifying PF16 as a key scaffold and uncovering lineage-specific adaptations.

The paper determines the cryoEM structure of PdpC, an effector protein from Francisella tularensis, and shows its binding to host phospholipids, revealing insights into its role in virulence.

The paper determines the cryo-EM structure of native Trichomonas vaginalis doublet microtubules, identifying parasite-specific proteins and a potential drug molecule.

This paper develops a rapid, specific, and reusable DNA aptamer-based biosensor for detecting SARS-CoV-2 spike protein, demonstrating high sensitivity and structural insights through cryo-EM.

This paper shows that ethanolamine utilization via BMCs is essential for Fusobacterium nucleatum virulence, as BMC formation is regulated by a TCS and disruption reduces pathogenicity in a mouse model.

This study uses cryoID to reconstruct the native PLP synthase complex from Methanosarcina acetivorans lysate, revealing a homo-dodecamer structure and absence of a previously proposed supracomplex.

This paper introduces spIsoNet, a deep-learning tool that improves 3D reconstruction isotropy and particle alignment in cryoEM by addressing the preferred orientation problem without altering specimen preparation.

This study uses cryoET and deep learning to reveal structural differences in the tegument and envelope of EBV and KSHV virions compared to other herpesviruses.

The paper identifies five lethal and two live attenuated HCMV mutants by mutating pp150-capsid interactions, revealing critical residues for viral replication.

This study presents cryoEM structures of SARS-CoV-2 Nsp15 bound to dsRNA, revealing how it recognizes and cleaves poly(U) tracts to evade host immunity.

TomoNet is a cryoET software pipeline with automatic particle picking and subtomogram averaging, achieving high-resolution 3D structures from flexible lattices.

The paper presents the near-atomic structure of the aquareovirus core and reveals structural differences that suggest a mechanism for transcription initiation in multilayered reoviruses.

This study discovers that native MCC from Leishmania tarentolae forms filaments, determines its structure, and proposes a new model for its catalytic mechanism.

The paper reports cryoEM structures of endogenous 2-oxoglutarate and pyruvate dehydrogenase complexes from bovine kidney, revealing structural differences and insights into their organization.

The paper reports cryo-EM structures of Tetrahymena CST and PolPrim in complex with telomerase, revealing their structural basis for DNA synthesis at telomeres.

The paper identifies the in situ structure of GABAAR in hippocampal inhibitory synapses and describes its hierarchical organization, revealing mesophasic self-organization that balances stability and flexibility.

This study compares the capsid-binding patterns of pp150 in human and murine cytomegaloviruses using cryoEM, revealing structural differences and functional implications for using MCMV as a model for HCMV.