Stephan Uphoff

RecA structure kinetics determine SOS dynamics and predict spontaneous cell death

The paper shows RecA* structure dynamics predict spontaneous cell death in E. coli by linking SOS response magnitude to DNA repair kinetics.

Single-molecule imaging of DNA repair and cytoplasmic rigidification in intracellular bacteria

This study uses live-cell single-molecule tracking to measure DNA repair activity in intracellular E. coli and links it to metabolic and biophysical changes during macrophage infection.

A systematic identification of resistance determinants to antisense antibiotics suggests adaptation strategies dependent on the delivery peptide

This study identifies resistance determinants to antisense antibiotics by analyzing mutations in gram-negative pathogens exposed to CPP-PNA conjugates, revealing adaptation strategies and resistance mechanisms.

Replication-coupled search positions MutH for strand incision in DNA mismatch repair

The paper investigates how MutH is recruited to DNA for strand incision in E. coli MMR by analyzing its search dynamics in live cells.

Real-time visualisation of the intracellular dynamics of Shigella virulence plasmid

This study visualizes the intracellular dynamics of Shigella sonnei's virulence plasmid pINV in real-time using fluorescent tagging.

Selective interaction of the protein SMCHD1 with specific chromatin regions is governed by the loading factor LRIF1 and SMCHD1 ATPase activity

This study investigates SMCHD1's chromatin interactions and shows LRIF1 and ATPase activity are critical for its selective binding and epigenetic silencing functions.

A simple regulatory network coordinates a bacterial stress response in space and time

The paper uses time-resolved single-cell imaging to study the Escherichia coli oxidative stress response, revealing simple regulatory logic in gene expression dynamics.

Imaging LexA degradation in cells explains regulatory mechanisms and heterogeneity of the SOS response

The paper uses single-molecule imaging to study LexA degradation in E. coli, showing it causes SOS response heterogeneity and spontaneous pulses that improve survival.