Stephanie A. Gaglione

Activation-dependent lentiviruses enable antigen-specific T cell expansion and transduction

This paper develops 4-1BB-targeting lentiviral vectors to selectively expand and transduce antigen-specific T cells, enhancing their function in cancer immunotherapy.

Scalable TCR synthesis and screening enables antigen reactivity mapping in vitiligo

The paper introduces a scalable TCR synthesis and screening method, TCRAFT, to map antigen reactivity in vitiligo and pancreatic ductal adenocarcinoma.

Interrogating antiviral antibody responses with multiplexed, high-throughput serum assays

This paper develops ARCADE, a high-throughput assay to profile antiviral antibody responses, showing mRNA vaccines elicit broader and stronger responses than adenovirus vector vaccines.

Peptide-MHC-targeted retroviruses enable in vivo expansion and gene delivery to tumor-specific T cells

This study develops pMHC-targeted retroviruses for in vivo gene delivery to tumor-specific T cells, enhancing their expansion and anti-tumor activity in mouse models.

Deep mapping of the TCR-antigen interface using pMHC-pseudotyped viruses and yeast display

The paper introduces VelociRAPTR, a method to map TCR-antigen interactions by combining yeast-displayed TCR libraries with pMHC-displaying virus-like particles, revealing biased recognition patterns and enabling TCR engineering.

Model-guided design of microRNA-based gene circuits supports precise dosage of transgenic cargoes into diverse primary cells

The paper designs and characterizes ComMAND, a microRNA-based gene circuit that precisely controls transgene expression in diverse primary cells.