Nicholas Z. Lue

Genetic landscape of DNMT3A R882H reveals mechanism of aberrant oligomerization

This study identifies two mechanisms that rescue DNMT3A R882H activity by analyzing combinatorial mutations and showing R882H promotes aberrant oligomerization.

DNA methylation insulates genic regions from CTCF loops near nuclear speckles

The paper investigates how DNA methylation affects CTCF-mediated chromatin loops and gene expression by using DNMT1 inhibition and targeted protein degradation.

Structural insights into transcriptional regulation by the helicase RECQL5

This study determines the structure of RECQL5-bound Pol II elongation complexes using cryo-EM, revealing how RECQL5 stabilizes Pol II and modulates its translocation state to reduce transcription stress.

Activity-based CRISPR Scanning Uncovers Allostery in DNA Methylation Maintenance Machinery

This study uses activity-based CRISPR scanning to identify allosteric sites in DNMT1 and UHRF1, revealing gain-of-function mutations that enhance DNA methylation activity.

Polycomb-lamina antagonism partitions heterochromatin at the nuclear periphery

The paper develops LIMe-Hi-C to study how Polycomb repressive complex 2 and lamina association regulate heterochromatin sub-compartments.

Profiling the Landscape of Drug Resistance Mutations in Neosubstrates to Molecular Glue Degraders

This study identifies drug resistance mutations in neosubstrates of molecular glue degraders using CRISPR-suppressor scanning and reveals common resistance mechanisms.