Author

Ceejay Lee

Broad InstituteORCID 0000-0002-4128-9328Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 5 Organism:human· 5 Assay:CRISPR screen· 2 Research Focus:drug response / drug discovery· 2 Research Focus:gene regulatory network inference· 2 Assay:ATAC-seq· 1 Assay:DNA methylation· 1 Research Focus:perturbation response prediction· 1

Frequent Co-Authors

Brian B. LiauiD· 5 shared Hui Si KwokiD· 4 shared Pallavi M. GosaviiD· 3 shared Allison P. SiegenfeldiD· 2 shared Amanda L. WaterburyiD· 2 shared Eunju NamiD· 2 shared Haibin MaoiD· 2 shared Hanjie JiangiD· 2 shared

Activity by Year

2023-2025 · peak 4/yr

Co-Author Collaboration Network

10 top collaborators · 39 mutual co-author links

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5 shared papersMolecular Biology

Brian B. LiauORCID

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Author profile →

5 papers

Recent·Most cited

Coupling CRISPR Scanning with Targeted Chromatin Accessibility Profiling using a Double-Stranded DNA Deaminase

Heejin Roh, Simon P. Shen, Hu Yan, Hui Si Kwok, Allison P. Siegenfeld, Ceejay Lee, M. Lisandra Zepeda, C. Guo, Shelby A Roseman, Caroline Comenho, Vijay G. Sankaran, Jason Daniel Buenrostro, Brian B. Liau

This paper introduces TDAC-seq, a method combining CRISPR perturbations with chromatin accessibility profiling to study cis-regulatory elements at single-nucleotide resolution.

Nature Methods · Sep 11, 2025Peer-reviewedMolecular Biology

DNA methylation insulates genic regions from CTCF loops near nuclear speckles

Shelby A Roseman, Allison P. Siegenfeld, Ceejay Lee, Nicholas Z. Lue, Amanda L. Waterbury, Brian B. Liau

The paper investigates how DNA methylation affects CTCF-mediated chromatin loops and gene expression by using DNMT1 inhibition and targeted protein degradation.

eLife · Sep 3, 2025Peer-reviewedGenomics

Asymmetric Engagement of Dimeric CRL3KBTBD4 by the Molecular Glue UM171 Licenses Degradation of HDAC1/2 Complexes

Megan J. R. Yeo, Olivia Zhang, Xiaowen Xie, Eunju Nam, N. Connor Payne, Pallavi M. Gosavi, Hui Si Kwok, Irtiza Iram, Ceejay Lee, Jiaming Li, Nicholas J. Chen, Khanh Hung Nguyen, Hanjie Jiang, Zhipeng A. Wang, Kwangwoon Lee, , Ning Zheng

This paper identifies UM171 as a molecular glue that promotes degradation of HDAC1/2 complexes by inducing asymmetric interactions between KBTBD4 and HDAC1.

Nature · Feb 12, 2025Peer-reviewedMolecular Biology

KBTBD4 Cancer Hotspot Mutations Drive Neomorphic Degradation of HDAC1/2 Corepressor Complexes

Xiaowen Xie, Olivia Zhang, Megan J. R. Yeo, Ceejay Lee, Ran Tao, Stefan Andrew Harry, N. Connor Payne, Eunju Nam, Leena Paul, Yiran Li, Hui Si Kwok, Hanjie Jiang, Haibin Mao, Jennifer L. Hadley, Hong Lin, , Brian B. Liau

This study identifies how KBTBD4 cancer mutations drive neomorphic interactions with HDAC1/2, leading to CoREST degradation and tumor growth, and shows potential therapeutic strategies.

Nature · Feb 12, 2025Peer-reviewedMolecular Biology

Activity-based CRISPR Scanning Uncovers Allostery in DNA Methylation Maintenance Machinery

Kevin Chun-Ho Ngan, Samuel M. Hoenig, Hui Si Kwok, Nicholas Z. Lue, Pallavi M. Gosavi, David A. Tanner, Emma M Garcia, Ceejay Lee, Brian B. Liau

This study uses activity-based CRISPR scanning to identify allosteric sites in DNMT1 and UHRF1, revealing gain-of-function mutations that enhance DNA methylation activity.

eLife · Feb 10, 2023Peer-reviewedGenomics