Mass spectrometry of Mag-Net-enriched plasma extracellular vesicles from 86 C57BL/6J mice reveals 272 age-correlated proteins, sex-divergent trajectories, and an age clock with 2.75-month mean absolute error.

bioRxiv · Jul 28, 2026 · PreprintMolecular Biology✦ In-depth digest

Multimodal analysis of molecular remodeling in aging spleen identified global and cell type specific changes

Katarina Vlajic, Alison Luciano, Gennifer E. Merrihew, Carla Ruiz Sanchez, Michael E. Riffle, Kristine A. Tsantilas, Sahar Attar, Brian J. Beliveau, Mariya T. Sweetwyne, Michael J. MacCoss, Gary A. Churchill, Devin K. Schweppe

The paper identifies age-related molecular changes in mouse spleens using proteomics and transcriptomics, showing cell-type-specific remodeling and gender differences in post-transcriptional buffering.

bioRxiv · Apr 21, 2026 · PreprintSystems Biology

A framework for quality control in quantitative proteomics

Kristine A. Tsantilas, Gennifer E. Merrihew, Julia E. Robbins, Richard S. Johnson, Jea Park, Deanna L. Plubell, Jesse D. Canterbury, Eric L. Huang, Michael E. Riffle, Vagisha Sharma, Brendan MacLean, Josh Eckels, Christine C. Wu, Michael S. Bereman, Sandra E. Spencer Miko, , Michael J. MacCoss

The paper introduces a framework for quality control in quantitative proteomics, combining internal and external QC measures with tools for data analysis and deposition.

Journal of Proteome Research · Sep 9, 2024Peer-reviewedBiochemistry

Multi-tissue landscape of somatic mtDNA mutations indicates tissue specific accumulation and removal in aging

Mónica Sánchez-Contreras, Mariya T. Sweetwyne, Kristine A. Tsantilas, Jeremy A. Whitson, Matthew D. Campbell, Brendan F. Kohrn, Hyeon Jeong Kim, Michael J. Hipp, Jeanne Fredrickson, Megan M. Nguyen, James B. Hurley, David Joseph Marcinek, Peter S. Rabinovitch, Scott R. Kennedy

The paper detects age-related tissue-specific accumulation of mtDNA mutations in mice and shows oxidative damage mutations are reduced with treatment.

eLife · Feb 17, 2023Peer-reviewedGenomics

A mutational gradient drives somatic mutation accumulation in mitochondrial DNA and influences germline polymorphisms and genome composition

Mónica Sánchez-Contreras, Mariya T. Sweetwyne, Brendan F. Kohrn, Kristine A. Tsantilas, Michael J. Hipp, Elizabeth K. Schmidt, Jeanne Fredrickson, Jeremy A Whitson, Matthew D Campbell, Peter S. Rabinovitch, David Joseph Marcinek, Scott R. Kennedy

The paper identifies a mutational gradient in mtDNA that correlates with replication and influences mutation accumulation and genome composition.

Nucleic Acids Research · Sep 22, 2021Peer-reviewedGenomics