Gregory R. Keele

Circulating extracellular vesicles in plasma carry accessible molecular signatures of aging in mice

Mass spectrometry of Mag-Net-enriched plasma extracellular vesicles from 86 C57BL/6J mice reveals 272 age-correlated proteins, sex-divergent trajectories, and an age clock with 2.75-month mean absolute error.

Genetic architecture of the murine serum metabolome reveals carboxyl esterases as master regulators of circulating fatty acid metabolism

The paper identifies genetic regulators of the murine serum metabolome, highlighting carboxyl esterases as key controllers of fatty acid metabolism.

A population-scale red blood cell proteome reveals genetically encoded aging clocks predictive of hemolysis and blood donor activity

This paper characterizes the RBC proteome across 13,091 donors, identifies genetic regulators of molecular aging, and shows that aging clocks predict hemolysis, transfusion outcomes, and donor activity.

Deep Red Blood Cell Proteome Defines the Band 3 N-Terminus Interactome as a Regulator of Hypoxic Adaptation via BLVRB-Dependent S-Nitroso Transfer

The paper defines the Band 3 N-terminus interactome in red blood cells, revealing its role in hypoxic adaptation through BLVRB-dependent S-nitroso transfer and metabolic remodeling.

Structural divergence of G6PD variants shapes red blood cell storage and transfusion performance

The paper investigates how G6PD variants affect red blood cell storage, transfusion performance, and recipient physiology using multiple datasets and experimental models.

Genetic dissection of tissue composition in genetically diverse mouse populations

The paper uses single-cell RNAseq data to analyze genetic factors influencing cell-type composition in mouse tissues, identifying heritability and QTLs associated with cell-type abundance.

Caffeine Impairs Red Blood Cell Storage Quality by Dual Inhibition of ADORA2b Signaling and G6PD Activity

Caffeine consumption and ADORA2b genetic variants impair RBC metabolism and transfusion outcomes through dual mechanisms, highlighting a modifiable interaction for precision transfusion strategies.

Integral Synthesis and Clearance Analysis via DIA (ISDia) Reveals Diverse Protein Abundance Regulation during Endoplasmic Reticulum Stress

ISDia tracks protein synthesis and clearance during ER stress, revealing PERK-dependent and independent regulatory mechanisms across subcellular compartments.

Expanding the Landscape of Aging via Orbitrap Astral Mass Spectrometry and Tandem Mass Tag (TMT) Integration

This study uses Orbitrap Astral mass spectrometry with TMT to analyze proteomic changes in mouse brain and kidney tissues across age and sex, revealing tissue-specific and non-linear age-related protein dynamics.

Genetic architecture of the red blood cell proteome in genetically diverse mice reveals central role of hemoglobin beta cysteine redox status in maintaining circulating glutathione pools

The paper identifies genetic factors influencing red blood cell proteome and oxidative stress in mice, highlighting hemoglobin cysteine redox status as critical for glutathione regulation.

Ferroptosis regulates hemolysis in stored murine and human red blood cells

This study identifies Steap3 as a regulator of ferroptosis-like lipid peroxidation in stored RBCs, linking genetic variants to hemolysis in mice and human blood donors.

Cell morphology QTL reveal gene by environment interactions in a genetically diverse cell population

The paper identifies cell morphology quantitative trait loci (cmQTL) in fibroblasts exposed to arsenic, revealing gene-environment interactions and genetic regulation of oxidative stress responses.

Global and tissue-specific aging effects on murine proteomes

The paper analyzes proteomic changes across 10 tissues in mice at different ages, finding age-related increases in immune proteins and tissue-specific alterations in protein complexes.

Genetic dissection of the pluripotent proteome through multi-omics data integration

This study integrates multi-omics data from mouse embryonic stem cells to identify genetic drivers of proteome variability and uncover regulatory mechanisms in pluripotency.

The Trouble with Triples: Examining the Impact of Measurement Error in Mediation Analysis

This paper shows that measurement error in mediation analysis can lead to incorrect causal inferences in genetic studies, even when no true mediation exists.

Which mouse multiparental population is right for your study? The Collaborative Cross inbred strains, their F1 hybrids, or the Diversity Outbred population

The paper evaluates the Collaborative Cross, Diversity Outbred, and CC-RIX populations for genetic analysis tasks, finding CC and CC-RIX better for heritability estimates and DO better for detecting small-effect loci in polygenic traits.

Genetic mapping of multiple metabolic traits identifies novel genes for adiposity, lipids and insulin secretory capacity in outbred rats

QTLViewer: An interactive webtool for genetic analysis in the Collaborative Cross and Diversity Outbred mouse populations

QTLViewer provides an interactive webtool for QTL mapping and exploration in CC and DO mouse populations, enhancing accessibility to genetic analysis resources.

Multi-omics analysis identifies drivers of protein phosphorylation

The paper identifies genetic drivers of protein phosphorylation using multi-omics data from mouse tissues, revealing novel interactions and targets of PDK1 in obesity and diabetes.

A Bayesian model selection approach to mediation analysis

The paper introduces a Bayesian model selection approach for mediation analysis and demonstrates its effectiveness using genetic data from mice and human cell lines.

Transcriptome-wide Analyses of Adipose Tissue in Outbred Rats Reveal Genetic Regulatory Mechanisms Relevant for Human Obesity

The paper identifies 554 consensus genes in outbred rats and humans associated with body weight and BMI, revealing shared genetic regulatory mechanisms relevant for obesity.

Inferring cell-type-specific causal gene regulatory networks during human neurogenesis

The paper identifies cell-type-specific causal gene regulatory networks in human neurogenesis by analyzing mediation between chromatin accessibility and gene expression.

Genome-wide transcript and protein analysis reveals distinct features of aging in the mouse heart

The paper analyzes transcriptome and proteome data from aged mouse hearts to identify molecular changes associated with aging, revealing distinct biological processes and genetic loci influencing age-related changes.

Systems genetics uncovers microbe-lipid-host connections in the murine gut

This study identifies genetic links between gut microbiota, lipids, and host transcripts in mice, revealing key roles for Akkermansia muciniphila and ornithine lipids in microbe-host interactions.

Regulation of protein abundance in genetically diverse mouse populations

The paper analyzes liver proteomes across three genetically diverse mouse populations to understand how genetic variation influences protein abundance and its relationship to phenotypes.

An optogenetic switch for the Set2 methyltransferase provides evidence for rapid transcription-dependent and independent dynamics of H3K36 methylation

This study uses optogenetic control of Set2 in yeast to show rapid, transcription-dependent H3K36me3 deposition and transcription-independent, stochastic demethylation.

Integrative QTL analysis of gene expression and chromatin accessibility identifies multi-tissue patterns of genetic regulation

The paper identifies genetic regulators of gene expression and chromatin accessibility across multiple tissues using a genetically diverse mouse population and proposes a causal model for gene regulation.

Determinants of QTL mapping power in the realized Collaborative Cross

The paper evaluates QTL mapping power in the Collaborative Cross using real genomes and simulations, providing guidelines for experimental design.

Bayesian decision theoretic design of two-founder experimental crosses given diallel data

The paper develops a Bayesian decision theoretic framework for designing two-founder experimental crosses using diallel data to optimize QTL mapping experiments.

Diallel analysis reveals Mx1-dependent and Mx1-independent effects on response to influenza A virus in mice

The paper uses a diallel analysis in mice to study how host genetics affect response to influenza A virus, identifying Mx1-dependent and independent heritable effects on weight loss.