David Issadore

Microglia modulate concussion biomarkers and cognitive recovery in male mice

This study shows microglia influence concussion recovery and identifies EV miRNA biomarkers affected by microglial presence.

Open-source robotic chip-to-plate interface for high-throughput microfluidic generation of materials libraries

LMNOP-bot automates microfluidic material library generation, increasing throughput 50x and enabling precise, reproducible deposition into standard well plates.

Brain-derived extracellular vesicle microRNAs in Lewy body and Alzheimer's disease

This study identifies miRNA biomarkers in brain-derived extracellular vesicles from plasma to distinguish Lewy body disease from Alzheimer's disease with high accuracy.

Scalable flow synthesis of ultrasmall inorganic nanoparticles for biomedical applications via a confined impinging jet mixer

This paper develops a scalable method to produce ultrasmall inorganic nanoparticles for biomedical use using a confined impinging jet mixer.

Automated and parallelized microfluidic generation of large and precisely-defined lipid nanoparticle libraries

This paper presents an automated microfluidic platform for generating large, precisely-defined lipid nanoparticle libraries and evaluates their physicochemical and transfection properties.

Elucidating lipid nanoparticle properties and structure through biophysical analyses

This study uses biophysical methods to characterize lipid nanoparticle properties and predict their transfection efficiency.

High-throughput, multiplexed quantification, and sorting of single EVs at single-molecule level

This paper introduces BDEVS, a platform for high-throughput, multiplexed, and ultra-sensitive profiling of individual extracellular vesicles in plasma, demonstrating a 100x improvement in detecting EV subpopulations.

Multi-stage-mixing to control the supramolecular structure of lipid nanoparticles, thereby creating a core-then-shell arrangement that improves performance by orders of magnitude

This paper introduces multi-stage-mixing (MSM) to control lipid nanoparticle (LNP) supramolecular structure, creating a core-then-shell arrangement that significantly improves mRNA and DNA-LNP performance.

Single-Mitochondrion Sequencing Uncovers Distinct Mutational Patterns and Heteroplasmy Landscape in Mouse Astrocytes and Neurons.

The paper analyzes mtDNA SNVs in single mitochondria from mouse neurons and astrocytes, revealing cell-type-specific mutation patterns and purifying selection.