Mohamad‐Gabriel Alameh

Impact of RNA modification and IL-12 boosts on mRNA-LNP vaccine-elicited CD8+ T cell responses

This study compares CD8+ T cell and antibody responses in non-human primates using different ratios of modified and unmodified mRNA-LNP vaccines with IL-12 boosting.

Evaluation of an H5 influenza virus mRNA-lipid nanoparticle (LNP) vaccine in lactating dairy cows

This study evaluates an H5 mRNA-LNP vaccine in lactating dairy cows, finding it reduces viral replication and disease in mammary glands.

Immunotherapy restores therapeutic efficacy of fecal microbiome transplants to treat Clostridioides difficile

This study shows that IL-10 signaling deficiency impairs FMT engraftment and C. difficile resolution, and that IL-10 immunotherapy can restore FMT efficacy in mice.

Protective pan-betacoronavirus neutralizing antibodies by vaccination

Epitope-focused S2 stem-helix nanoparticle immunogens elicit protective broadly neutralizing antibodies against diverse betacoronaviruses in non-human primates, establishing a framework for pan-betacoronavirus vaccines.

Generation and characterization of a novel MHC-II tetramer for tracking and characterization of toxin B-specific CD4+ T cell responses

The paper develops an MHC-II tetramer to track TcdB-specific CD4+ T cells in mice, validating its use in identifying Tfh cells and generating toxin-specific T cells.

Lipid nanoparticle co-delivery of mRNA and a small molecule drug for oral cancer chemoimmunotherapy

This paper develops a lipid nanoparticle co-delivering mRNA and a small molecule drug to treat oral cancer by enhancing chemotherapy and reshaping the tumor microenvironment.

Adenosine deaminase co-immunization reverses age-associated immunosenescence by restoring germinal center T follicular helper cell function

This study shows that co-immunizing with adenosine deaminase and a DNA vaccine improves T follicular helper cell function and antibody quality in aged mice, enhancing protection against C. difficile infection.

Plug-and-play assembly of biodegradable ionizable lipids for potent mRNA delivery and gene editing in vivo

This study develops a combinatorial chemistry approach to create biodegradable ionizable lipids for efficient mRNA delivery and gene editing in vivo.

Bioinspired Oxidized mRNA Lipid Nanoparticles for Ex Vivo Engineering of Chimeric Antigen Receptor Macrophages Targeting Solid Tumors

This paper develops a bioinspired oxidized lipid nanoparticle platform for mRNA-based engineering of CAR-Ms to target solid tumors, showing improved delivery and tumor-killing efficacy.

Correction of a recurrent pathogenic variant in methylmalonic acidemia using adenine base editing

This study corrects a common pathogenic variant in MMA using adenine base editing in hepatocytes, showing efficient in vitro editing with minimal off-target effects.

Investigating the Impact of Hypoxia and Syncytialization on Lipid Nanoparticle-Mediated mRNA Delivery to Placental Cells

This study evaluates how hypoxia affects lipid nanoparticle-mediated mRNA delivery to placental cells and shows that therapeutic mRNA can restore protein levels under hypoxic conditions.

Automated and parallelized microfluidic generation of large and precisely-defined lipid nanoparticle libraries

This paper presents an automated microfluidic platform for generating large, precisely-defined lipid nanoparticle libraries and evaluates their physicochemical and transfection properties.

Clostridioides difficile toxin A and toxin B inhibit toxin-specific adaptive immune responses through glucosyltransferase-dependent activity

This study shows that C. difficile toxins A and B inhibit their own antigen-specific immune responses via glucosyltransferase activity, contributing to recurrent infections.

Cytokine mRNA Delivery and Local Immunomodulation in the Placenta using Lipid Nanoparticles

This study uses lipid nanoparticles to deliver cytokine mRNA to placental cells, inducing anti-inflammatory responses and revealing dose-dependent polarization of macrophages.

An IL-12 mRNA-LNP adjuvant enhances mRNA vaccine induced CD8+ T cell responses

This study shows that adding IL-12 mRNA-LNPs to an mRNA vaccine enhances CD8+ T cell responses against model antigens and improves protection against infections and tumors.

The type I IFN-IL-27 axis promotes mRNA vaccine-induced CD8+ T cell responses

This study shows that IL-27, induced by mRNA-LNP vaccines, enhances CD8+ T cell responses through type I IFN signaling, improving vaccine efficacy against pathogens and tumors.

Improved specificity and efficiency of in vivo adenine base editing therapies with hybrid guide RNAs

This study evaluates hybrid guide RNAs to improve the specificity and efficiency of adenine base editing therapies for genetic disorders.

Epigenome Editing Durability Varies Widely Across Cardiovascular Disease Target Genes

This study evaluates the durability of epigenome editing for reducing gene expression in cardiovascular disease targets, finding durable effects for PCSK9 but not for ANGPTL3 or AGT.