Matthias Stadtfeld

KAT6 and KAT7 actively maintain mitotic chromatin composition and acetylation to preserve developmental competence

This study shows KAT6 and KAT7 maintain mitotic chromatin acetylation and composition, supporting postmitotic transcriptional memory and developmental competence in stem cells.

Developmental rewiring of EHMT1 function during human cortical neurogenesis

This study shows EHMT1's functional rewiring during human cortical neurogenesis, revealing its shift from restraining pluripotency to supporting neuronal programs and its resilience to prolonged depletion.

CTR1-mediated copper uptake orchestrates metabolic-epigenetic regulation of pathogenic TH17 cells in autoimmune disease

This study shows that CTR1-mediated copper uptake is essential for pTH17 cell differentiation and function in autoimmune disease by linking copper homeostasis to metabolic and epigenetic regulation.

A mouse organoid platform for modeling cerebral cortex development and cis-regulatory evolution in vitro

The paper develops a mouse cortical organoid platform to study cis-regulatory evolution and identifies genes with dynamic allelic imbalances during neurogenesis.

Genetic variation modulates susceptibility to aberrant DNA hypomethylation and imprint deregulation in naive pluripotent stem cells

The paper identifies genetic variants that influence DNA methylation stability and imprint deregulation in naive pluripotent stem cells across different mouse strains.

Coordinated repression of totipotency-associated gene loci by histone methyltransferase EHMT2 through binding to LINE-1 regulatory elements

The paper identifies EHMT2's role in repressing totipotency-associated gene loci in mESCs by binding to LINE-1 elements and modulating gene expression during the mESC-to-2CLC transition.

Transcriptional remodeling by OTX2 directs specification and patterning of mammalian definitive endoderm

This study identifies OTX2 as a critical regulator of definitive endoderm specification and gut patterning through chromatin remodeling and signaling pathway regulation.

Systematic mapping and modeling of 3D enhancer-promoter interactions in early mouse embryonic lineages reveal regulatory principles that determine the levels and cell-type specificity of gene expression

A bipartite element with allele-specific functions safeguards DNA methylation imprints at the Dlk1-Dio3 locus

The paper identifies a bipartite imprinting control region at the Dlk1-Dio3 locus that maintains imprint stability through allele-specific regulation of DNA methylation and chromatin structure.

Juvenile hormones direct primordial germ cell migration to the embryonic gonad

This study shows juvenile hormones (JHs) are necessary and sufficient for primordial germ cell (PGC) migration in Drosophila, with similar roles observed for retinoic acids in mammals.

The BTB transcription factors ZBTB11 and ZFP131 maintain pluripotency by pausing POL II atpro-differentiation genes

A CRISPR screen identifies ZBTB11 and ZFP131 as factors maintaining pluripotency by pausing POL II at pro-differentiation genes.

EpiMethylTag simultaneously detects ATAC-seq or ChIP-seq signals with DNA methylation

EpiMethylTag combines ATAC-seq or ChIP-seq with bisulfite conversion to simultaneously assess chromatin accessibility/TF binding and DNA methylation.

Context-dependent requirement of H3K9 methyltransferase activity during cellular reprogramming to iPSCs

This study shows that reducing EHMT1/2 activity during reprogramming with ascorbic acid impairs iPSC formation and viability, highlighting the role of H3K9 methylation in reprogramming efficiency.

KLF4 is involved in the organization and regulation of pluripotency-associated 3D enhancer networks

This study investigates the role of KLF4 in chromatin reorganization and 3D enhancer rewiring during reprogramming of mouse embryonic fibroblasts to pluripotent stem cells.