Yuanyuan Hou

"Self-inactivating" rabies viruses are susceptible to loss of their intended attenuating modification

The paper shows that a modified rabies virus intended for noncytotoxic neuronal tracing lost its modification due to mutations, leading to paradoxical results, and that the modified virus requires exogenous protease for efficient spread.

Single-deletion-mutant, third-generation rabies viral vectors allow nontoxic retrograde targeting of projection neurons with greatly increased efficiency

This paper introduces third-generation rabies viral vectors with single deletions that enable more efficient, non-toxic retrograde targeting of projection neurons compared to previous generations.

Long-term labeling and imaging of synaptically-connected neuronal networks in vivo using nontoxic, double-deletion-mutant rabies viruses

This paper introduces a non-toxic rabies virus system for long-term in vivo imaging of neuronal networks, showing minimal toxicity and normal visual response properties over 10 weeks.

Prime editing primarily induces undesired outcomes in mice

In mouse zygotes, prime editing installed 10 modifications across 6 genes, but undesired products such as large deletions outnumbered intended edits, traced to PE3 double-nicking.