Christophe Hirtz

SPEAR: Spatial Epitranscriptomic Analysis by Mass Spectrometry Reveals Localized RNA Modification Remodeling During Glioma Progression

SPEAR combines laser microdissection and LC-MS/MS to enable spatial epitranscriptomic profiling of FFPE tissue, revealing RNA modification changes in glioma progression.

Integrated tear proteomics define the molecular blueprint of corneal epithelial repair

This study identifies conserved and species-specific molecular pathways in tear film during corneal wound healing in humans and mice.

Reversible multiscale corneal sexual dimorphism orchestrates neuro-epithelial coupling and tear proteome composition

This study shows that sex hormones influence corneal graft rejection risk by modulating tear composition, epithelial dynamics, and sensory function, with androgen treatment reversing these sex-linked differences.

Loss of tRNA uridine thiolation affects mRNA translation, protein production, and sulfur-compound metabolism in Arabidopsis

RABV L protein plays a role in immune escape through its methyltransferase activity

This study shows that the K-D-K-E motif in RABV L protein's methyltransferase domain is crucial for immune evasion by altering viral and host RNA methylation, increasing sensitivity to interferon, and reducing pathogenicity.

Temporal analysis of tear fluid proteome reveals critical corneal repair events after photorefractive surgery

This study uses proteomic analysis to track tear fluid changes after PRK, identifying key molecular events in corneal repair and potential biomarkers for healing.

Decoding the molecular complexity governing corneal wound closure in vivo

This study investigates corneal wound healing using multi-omics analyses, identifying molecular factors and epithelial plasticity mechanisms critical for tissue regeneration.

Dual CRALBP isoforms unveiled: iPSC-derived retinal modelling and AAV2/5-RLBP1 gene transfer raise considerations for effective therapy

The paper models RLBP1-related retinal diseases using iPSC-derived RPE and validates a gene replacement strategy, identifying a novel CRALBP isoform with functional significance.

Inherited mitochondrial dysfunction triggered by OPA1 mutation impacts the sensory innervation fibre identity, functionality and regenerative potential in the cornea

This study shows that OPA1 mutation causes corneal innervation issues, including reduced sensitivity and impaired regeneration, despite normal epithelial function.

Enabling population protein dynamics through Bayesian modeling

This paper introduces a Bayesian modeling approach to capture population protein dynamics using cohort data, showing its ability to handle inter-individual variability.

Modeling the simultaneous dynamics of proteins in blood plasma and the cerebrospinal fluid in human in vivo

This study develops a 3-compartment model to simultaneously analyze protein dynamics in blood plasma and cerebrospinal fluid, including a hidden CNS compartment, and applies it to 69 proteins from a single individual.

A candidate reference method for the quantification of α-synuclein in cerebrospinal fluid using an SI traceable primary calibrator and multiple reaction monitoring

This paper develops a reference method for quantifying α-synuclein in CSF using SI-traceable calibration and MRM, showing linearity across three orders of magnitude.

E4F1 COORDINATES PYRUVATE METABOLISM AND THE ACTIVITY OF THE ELONGATOR COMPLEX TO ENSURE TRANSLATION FIDELITY DURING BRAIN DEVELOPMENT

A single short reprogramming early in life improves fitness and increase lifespan in old age

A single short reprogramming early in life rejuvenates cell physiology, improves body composition, tissue fitness and increases lifespan in elderly.

In Vivo Large Scale Mapping Of Protein Turnover In The Human Cerebrospinal Fluid

The paper introduces a proteomics framework to map protein turnover in human cerebrospinal fluid, identifying turnover rates for nearly 200 proteins and revealing relationships between turnover and protein origin.