Marcel P. Bruchez

Optimization of the fluorogen-activating protein tag for quantitative protein trafficking and co-localization studies in S. cerevisiae

The paper optimizes fluorogen-activating proteins (FAPs) for use in S. cerevisiae to enable quantitative endocytic studies and co-localization analysis of proteins.

Ca2+ and cAMP open differentially dilating synaptic fusion pores

The paper shows that cAMP signaling opens dilating synaptic fusion pores for releasing large proteins, while Ca2+ triggers narrower pores for smaller cargo.

Telomeric 8-oxoguanine drives rapid premature senescence in the absence of telomere shortening

This study shows that telomeric 8-oxoguanine induces premature senescence without telomere shortening by causing replication stress and dysfunction.

Temporally and Spatially Partitioned Neuropeptide Release from Individual Clock Neurons

The paper shows that Drosophila clock neurons release neuropeptides from different compartments at different times, with somatic release controlled by IP3 and terminal release by Ca2+.

Quantitative synapse analysis for cell-type specific connectomics

The paper develops genetically-encoded reagents for cell-type specific synapse labeling and connectivity analysis in mouse brain tissue, revealing PV inputs as the dominant inhibitory source onto L2 pyramidal neurons.