Mark C. Leake

This study identifies Ktd1 as a phospho-regulated member of the Dup240 family essential for defense against the K28 killer toxin in yeast.

This study shows ESR1 fusions form chromatin-anchored condensates that remodel nuclear architecture, supporting endocrine resistance and tumour progression.

HaloUMI provides an automated tool for analyzing microbial inhibition assays, improving accuracy by accounting for irregular zones and varying microbial density.

The paper investigates how the 2A protein interacts with the RNA genome of cardioviruses to activate ribosomal frameshifting through structural changes in the RNA.

Live E. coli gyrase clusters hold excess GyrB (B:A 2.5:1), show hexanediol-sensitive liquid-like turnover, and docking suggests GyrB bridges heterotetramers.

This study uses single-molecule super-resolution microscopy to analyze the in vivo dynamics of DNA gyrase in live E. coli, revealing its association with replisomes and highlighting the importance of studying all subunits of enzyme complexes.

This study shows that varying the number of binding motifs in a disordered protein affects pyrenoid assembly and algal fitness.

This paper introduces a fluorescence de-quenching assay to study detergent-induced membrane remodeling, showing Triton X-100 causes morphological transitions at sub-critical concentrations.

This study investigates the conformational transitions of Rep helicase using single-molecule techniques, revealing two intermediate states influenced by salt concentration.

The paper investigates the molecular architecture of the Fps1 aquaglyceroporin gating system in yeast, revealing that Rgc2 functions as a homodimer and Rgc1 influences its dynamics and stability.

This study uses atomic simulations to show that HU binds DNA through a multi-step process involving weak electrostatic interactions, protein reorientation, and DNA flexibility, ensuring specific binding at target sites.

SlimVar enables in vivo single-molecule tracking of chromatin regulators in Arabidopsis thaliana root cells, revealing dynamic protein assemblies during FLC silencing.

This study investigates the role of VIN3 and VRN5 VEL polymerization domains in cold-induced epigenetic silencing of FLC in Arabidopsis, showing distinct functional contributions.

The paper shows that aggresomes in E. coli store mRNA under stress, aiding rapid recovery by protecting mRNA from degradation and facilitating translation reactivation.

This paper develops a DNA scaffold method for 5' labeling of SARS-CoV-2 RNA pseudoknots to study their conformational dynamics using smFRET.

This study shows that macrophage-derived ROS induce Salmonella aggresome formation, leading to antibiotic persistence through dormancy and resuscitation capability.

The paper introduces iPAR, a fluorescent reporter for studying cytoplasmic protein aggregation in yeast, showing aggregates are mobile and not inherited by daughter cells.

The paper identifies a conserved linker protein from Chlorella that can phase separate Rubisco in Chlamydomonas and some plants, enabling cross-species pyrenoid formation.

This study investigates the in vivo properties of Arabidopsis FCA condensates involved in RNA 3' processing, revealing their modular structure and interactions with FLL2 and RNA.

The paper introduces COMBI-Tweez, a tool combining optical and magnetic tweezers with fluorescence microscopy to study the structural dynamics of single chiral biopolymers like DNA under mechanical forces.

The paper uses single-molecule fluorescence microscopy to study SpoIIE dynamics in Bacillus subtilis during sporulation, revealing changes in stoichiometry and mobility that enable stage-specific functions.

This study uses single-molecule tracking to show EGFR homoclusters pre-activate and form heteroclusters with HER2 after EGF binding, with a peak EGFR-to-EGF ratio of 2.

The paper develops a super-resolution microscopy system for rapid molecular tracking in live tissues and demonstrates its application to chemokine molecules in collagen and lymph node tissues.

The paper determines the stoichiometry and dynamics of LukS/LukF-hC5aR complexes in living cells, finding tetrameric complexes that dissociate upon pore formation.