This study identifies the conserved bridging domain on HCV E1E2 as a target for neutralizing antibodies from diverse lineages, showing additivity with neutralizing face antibodies.

Cell Reports · Aug 1, 2026Peer-reviewedImmunology

Structural and biochemical insights into HCV envelope proteins for germline-targeting vaccines

Itai Yechezkel, Hila Maymon, Ariel Tennenhouse, Fang Chen, Haneen Tarabih, Jenna Weisz, Rina Fraenkel, Sarel Jacob Fleishman, Mansun Law, Netanel Tzarum

This study identifies structural features of HCV E2 glycoprotein recognized by germline B cell receptors and evaluates strategies to enhance germline antibody interactions.

bioRxiv · May 1, 2026 · PreprintBiochemistry

Structural and genetic signatures of two classes of HCV E2 neutralizing face antibodies from non-human primates immunized with a recombinant E1E2

Yen Thi Kim Nguyen, Fang Chen, Erick Giang, Swati Saha, Lynn A. Ueno, Christopher Chen, Corey T. Watson, Netanel Tzarum, Ian A. Wilson, Mansun Law, Robyn L. Stanfield

This study identifies two classes of HCV E2 neutralizing antibodies from non-human primates immunized with a recombinant E1E2, showing similarity to human antibodies.

npj Vaccines · Apr 21, 2026Peer-reviewedImmunology

Native-like soluble E1E2 glycoprotein heterodimers on self-assembling protein nanoparticles for hepatitis C virus vaccine design

Linling He, Yi-Zong Lee, Yi-Nan Zhang, Maddy L. Newby, Benjamin M. Janus, Fabrizio G. Gonzalez, Garrett Ward, Connor DesRoberts, Shr-Hau Hung, Erick Giang, Joel D. Allen, Liudmila Kulakova, Eric A. Toth, Thomas R. Fuerst, Mansun Law, , Jiang Zhu

The paper designs, characterizes, and tests a soluble HCV E1E2 heterodimer and self-assembling nanoparticles for vaccine development.

Nature Communications · Feb 11, 2026Peer-reviewedMicrobiology

scFPC-DE: Robust Differential Expression Analysis Along Single Cell Trajectories via Functional Principal Component Analysis

Ricardo J. López Candelaria, Yu Qian, Fang Chen, Mansun Law, Yun Zhang, Xing Qiu

scFPC-DE identifies temporally differentially expressed genes along single-cell trajectories by modeling gene expression as functions of pseudotime and using functional principal component analysis to capture shared covariance structures.

bioRxiv · Nov 3, 2025 · PreprintBioinformatics

B cell transcriptomics reveals lasting dysregulation and rapid decline of protective memory after hepatitis C cure

Fang Chen, Erick Giang, Padmaja Natarajan, Tony S. Mondala, Steven Robert Head, Sing Chau Lau, Aishwarya Sundaresan, Liudmila Kulakova, Xiaohe Lin, Jasneet Aneja, Raiees Andrabi, Yun Zhang, Richard H. Scheuermann, Eric A. Toth, Thomas R. Fuerst, , Mansun Law

This study shows that B cells in hepatitis C patients retain dysregulated transcriptional profiles after cure, with sustained TNF signaling driving chronic inflammation and loss of protective memory.

bioRxiv · Oct 24, 2025 · PreprintImmunology

AIRR-C Human IG Reference Sets: curated sets of immunoglobulin heavy and light chain germline genes

Andrew M. Collins, Mats Ohlin, Martin Corcoran, James Heather, Duncan Ralph, Mansun Law, Jesús Martínez-Barnetche, Jian Ye, Eve Richardson, William S. Gibson, Oscar L. Rodriguez, Ayelet Peres, Gur Yaari, Corey T. Watson, William Lees

The paper curates high-quality human immunoglobulin germline gene reference sets to improve AIRR-seq analysis accuracy and facilitate immunoglobulin gene studies.

Frontiers in Immunology · Feb 9, 2024Peer-reviewedGenomics

Exploring protein orthogonality in immune space: a case study with AAV and Cas9 orthologs

Ana M. Moreno, Nathan D. Palmer, Fernando Alemán, Genghao Chen, Andrew Pla, Wei Leong Chew, Mansun Law, Prashant Mali

The paper explores immune orthogonality among protein orthologs by analyzing CRISPR-Cas9 and AAV serotypes, finding high immune orthogonality in Cas9 but not in AAVs.

bioRxiv · Jan 10, 2018 · PreprintSynthetic Biology