BEHAV3D-Explorer provides a user-friendly platform for analyzing 3D live-cell imaging data using natural language guidance to select analysis strategies and configure workflows.

bioRxiv · Nov 9, 2026 · PreprintBioinformatics

Regional specificity of neuronal cell types, functional connectivity, and cell type-specific correlation to brain disorders in the human ventral tegmental area

Anna van Regteren Altena, Bernardo de A.P.C. Maciel, Rachel M. Brouwer, Chen Ji Rong Jiang, Sara Seoane, Ravian L. van Ineveld, Jonne M Eggink, Tiziana M. Hey, Anne C. Rios, R. Jeroen Pasterkamp, Vanessa Donega, Martijn P. van den Heuvel, Onur Başak, Elly M. Hol

This study creates a transcriptomic atlas of the human ventral tegmental area, identifies cell types and their functional connectivity, and links them to psychiatric disorders and BMI.

bioRxiv · Jun 9, 2026 · PreprintNeuroscience

Cancer-myeloid cell invasive program in pediatric-type diffuse high-grade glioma

Cristian Ruiz-Moreno, Raphaël V U Collot, Thijs J M van den Broek, Ellen J. Wehrens, Nils Bessler, Gitanjali Dharmadhikari, Brigit M. te Pas, Ignacio L. Ibarra, Dennis Serge Metselaar, Mariëtte E.G. Kranendonk, Eelco Wieger Hoving, Jasper van der Lugt, Friso Calkoen, Anoek Zomer, Fabian Theis, , Hendrik G. Stunnenberg

This paper presents an integrative multimodal atlas of pediatric-type diffuse high-grade gliomas, identifying cancer-myeloid cell programs and distinct cancer cell states across multiple patients.

bioRxiv · Jan 25, 2026 · PreprintCancer Biology

De novo H3.3K27M-altered Diffuse Midline Glioma in human brainstem organoids to dissect GD2 CAR T cell function

Nils Bessler, Amber K. L. Wezenaar, Hendrikus CR Ariese, Celina Honhoff, Noëlle Dommann, Ellen J. Wehrens, Cristian Ruiz-Moreno, Thijs J M van den Broek, Raphaël V U Collot, Daan J. Kloosterman, Farid Keramati, Mieke M Roosen, Sam de Blank, Esmée J. van Vliet, Mario Barrera Román, , Anne C. Rios

This paper develops a human brainstem organoid model of H3.3K27M-altered DMG to study GD2 CAR T cell function and identify therapeutic targets.

Nature Cancer · Jan 5, 2026Peer-reviewedCancer Biology

BEHAV3D Tumor Profiler to map heterogeneous cancer cell behavior in the tumor microenvironment

Emilio Rios-Jimenez, Anoek Zomer, Raphaël V U Collot, Mario Barrera Román, Sandra F Archidona, Hendrikus Ariese, Ravian L. van Ineveld, Michiel Kleinnijenhuis, Nils Bessler, Hannah R. Johnson, Caleb A. Dawson, Anne C. Rios, María Alieva

BEHAV3D-TP is a computational framework for analyzing single-cell behavior in tumor microenvironments using intravital microscopy data, revealing migratory behaviors linked to specific TME components in diffuse midline glioma.

eLife · Oct 15, 2025Peer-reviewedCancer Biology

Single-cell spatial analysis of pediatric high-grade glioma reveals a novel population of SPP1+/GPNMB+ myeloid cells with immunosuppressive and tumor-promoting capabilities

Thijs J M van den Broek, Raoull Hoogendijk, Mariëtte E.G. Kranendonk, Julie A S Lammers, Akshaya L Krishnamoorthy, Ravian L. van Ineveld, Milo Molleson, Vasily O. Tsvetkov, Femke C. A. S. Ringnalda, Marc van de Wetering, Yan Su, John I. Bianco, Cristian Ruiz-Moreno, Mario G. Ries, Eelco Wieger Hoving, , Anoek Zomer

The paper uses single-cell spatial analysis to identify SPP1+/GPNMB+ myeloid cells in pediatric high-grade gliomas that suppress CD8+ T cells and promote tumor growth.

Neuro-Oncology Pediatrics · Jul 1, 2025Peer-reviewedCancer Biology

Hybrid supramolecular-covalent bioresin promotes cell migration and self-assembly in light-based volumetric bioprinted constructs

Marc Falandt, Paulina Núñez Bernal, Alessia Longoni, Maj‐Britt Buchholz, Pere Català, Klement Widmann, Mario Barrera-Roman, Jos Malda, Tina Vermonden, Anne C. Rios, Riccardo Levato

This paper introduces a hybrid biomaterial that enhances cell migration and self-assembly in bioprinted constructs, enabling complex tissue-like structures with improved vascular network formation.

bioRxiv · Jan 6, 2025 · PreprintBioengineering