Bénédicte Mascrez

EVOLUTIONARY CO-OPTION OF AN ANCESTRAL CLOACAL REGULATORY LANDSCAPE DURING THE EMERGENCE OF DIGITS AND GENITALS

The paper shows that the regulatory landscape controlling Hoxd gene expression in limbs was co-opted from an ancestral cloacal program in tetrapods.

CTCF-DEPENDENT INSULATION OF Hoxb13 AND THE HETEROCHRONIC CONTROL OF TAIL LENGTH

The paper investigates how CTCF sites in a DNA spacer delay Hoxb13 activation, affecting tail length by modulating its heterochronic expression.

Sequential And Directional Insulation By Conserved CTCF Sites Underlies The Hox Timer In Pseudo-Embryos

The paper shows that conserved CTCF sites create sequential insulation, controlling the timing of Hox gene activation in pseudo-embryos through chromatin extrusion and cohesin loading.

CONTEXT-DEPENDENT ENHANCER FUNCTION REVEALED BYTARGETED INTER-TAD RELOCATION

The paper shows that enhancer function depends on their chromatin context by relocating a distal limb enhancer into a proximal limb TAD, where it loses its distal specificity.

Sequential in-cis mutagenesis in vivo reveals various functions for CTCF sites at the mouse HoxD cluster

The paper uses in-cis mutations to study CTCF site functions in the mouse HoxD cluster, showing their roles in insulation, enhancer-promoter interactions, and TAD border formation.

MESOMELIC DYSPLASIAS ASSOCIATED WITH THE HOXD LOCUS ARE CAUSED BY REGULATORY REALLOCATIONS

The paper shows that mesomelic dysplasia caused by HOXD locus rearrangements results from ectopic HOXD13 expression due to regulatory reallocations.

HOX13-MEDIATED DBX2 REGULATION IN LIMBS SUGGESTS INTER-TAD SHARING OF ENHANCERS

The paper identifies HOX13-mediated regulation of DBX2 in limb development and shows enhancers can cross TAD boundaries to regulate genes in different domains.

Chromatin Topology And The Timing Of Enhancer Function At The HoxD Locus

The paper investigates how chromatin topology affects the timing of enhancer function at the HoxD locus, finding that enhancer sequences can overcome adverse chromatin architecture to activate genes.

A Complex Regulatory Landscape Involved In The Development Of External Genitals

The paper investigates how regulatory landscapes around the HoxD gene cluster are formed and function, finding that individual sequence deletions have minimal impact on transcription.

Constrained Transcriptional Polarity In The Organization Of Mammalian Hox Gene Clusters

The paper investigates the role of uniform transcriptional orientation in mammalian Hox gene clusters by engineering inversions and observing their impact on gene regulation.

Single-cell mRNA profiling reveals heterogeneous combinatorial expression of Hoxd genes during limb development

The paper analyzes single-cell transcriptomes of developing mammalian limb buds to show that Hoxd genes are expressed in specific combinations linked to cell types and follow a pseudotime trajectory during digit development.