Gyun Min Lee

Product-stabilized filamentation by human glutamine synthetase allosterically tunes metabolic activity

This paper shows human glutamine synthetase forms filaments stabilized by glutamine, reducing catalytic specificity for ammonia and linking product concentration to enzyme function.

Improving Recombinant Antibody Production Using FcBAR: An In Situ Approach to Detect and Amplify Protein-Protein Interactions

The paper introduces FcBAR, a method to detect protein-protein interactions in situ for recombinant proteins, and identifies factors that enhance rituximab production.

Multiplex genome editing eliminates the Warburg Effect without impacting growth rate in mammalian cells

This study eliminates the Warburg Effect in mammalian cells by knocking out lactate dehydrogenase and feedback regulators, maintaining growth rate while reducing lactate production.

Using targeted genome integration for virus-free genome-wide mammalian CRISPR screen

This paper develops a virus-free CRISPR screening platform for CHO cells, identifying 338 essential genes and 76 genes involved in ER stress.

A Chinese hamster transcription start site atlas that enables targeted editing of CHO cells

The paper revises transcription start site annotations in Chinese hamster genes using experimental data to improve gene engineering and glycosylation in CHO cells.

Genome-scale reconstructions of the mammalian secretory pathway predict metabolic costs and limitations of protein secretion