Tsung-Han S. Hsieh

The paper introduces a scalable Perturb-seq platform called FiCS Perturb-seq and releases X-Atlas/Orion, a large Perturb-seq atlas, to improve foundation model development through dose-dependent genetic effects.

The paper investigates how enhancer-promoter interactions and transcription are affected by acute loss of CTCF, cohesin, WAPL, and YY1, finding minimal effects on E-P interactions and gene expression despite 3D genome perturbations.

The paper shows cohesin residency at CARs determines chromatin loop positioning in budding yeast through loop extrusion and CAR-bound cohesin stopping.

The paper investigates how transcriptional regulatory elements influence the 3D organization of microTADs in mouse stem cells.

This study improves chromosome architecture mapping by using Micro-C to achieve nucleosome-resolution insights in human cells, revealing new looping interactions and structural patterns.

This study uses super-resolution imaging to reveal the 3D structure and organizing mechanism of accessible chromatin domains.

This study shows that removing tRNA genes from a chromosome does not broadly disrupt chromosome folding but affects local nucleosome positioning and SMC protein binding, as well as centromere and heterochromatin clustering.

This study identifies condensin-mediated chromatin condensation as a mechanism repressing transcription during quiescence in yeast and human cells.