Alexander Leitner

This paper introduces a XL-MS workflow combining DDA and PRM for quantitative characterization of protein-RNA interactions, applied to TDP-43 and FOX1 complexes.

This study shows that the NTD of TDP-43 binds UG-rich RNA on monomers, while oligomerization is essential for repressing cryptic exons.

The paper identifies complex protein network disruptions in human motile cilia from PCD patients using cryo-ET and proteomics, revealing differences from Chlamydomonas.

This study identifies HPCAL1 as a Ca2+-dependent regulator of AC8, revealing a new mechanism for cAMP signaling regulation.

This study identifies molecular determinants that guide DCAF specificity for CUL4B in the CRL4B E3 ubiquitin ligase complex through structural and biochemical analyses.

The paper compares proteomic and structural differences in cilia from PCD patients with DNAH5 mutations, showing patient-specific reductions in ciliary components and structural changes in the axoneme.

The paper shows that SNARE complex proteins inhibit NALCN channel activity and that reducing NALCN currents promotes cell survival in syntaxin-depleted cells.

The paper investigates protein interactions in cilia using CRISPR/CAS9, cryo-ET, and mass spectrometry, revealing a complex involving FAP263, FAP78, FAP184, and FAP151, and links it to dynein f phosphorylation.

This study shows TG2 binds more strongly to relaxed fibronectin fibers than stretched ones, revealing a mechano-regulated interaction mechanism.

The paper uses AlphaFold2 to predict structures for 65,484 human protein interactions, identifying high confidence models and disease-related mutations.

The paper examines how polyQ expansion affects HTT structure and phosphorylation, showing structural changes and altered phosphorylation patterns that influence HTT activity.

This paper summarizes current XL-MS practices, compares strategies, and proposes common protocols to establish best practice guidelines for accurate and reproducible results.

The paper identifies structural and kinetic mechanisms of COP9-Signalosome activation and Nedd8 deconjugation in cullin-RING ubiquitin ligase deneddylation.