Fly Cell Atlas: a single-cell transcriptomic atlas of the adult fruit fly

Hongjie Li; Jasper Janssens; Maxime De Waegeneer; Sai Saroja Kolluru; Kristofer Davie; Vincent Gardeux; Wouter Saelens; Fabrice P. A. David; Maria Brbić; Katina I. Spanier; Jure Leskovec; Colleen N. McLaughlin; Qijing Xie; Robert C. Jones; Katja Brueckner; Jiwon Shim; Sudhir Gopal Tattikota; Frank Schnorrer; Katja Rust; Todd G. Nystul; Zita Carvalho-Santos; Carlos Ribeiro; Soumitra Pal; Sharvani Mahadevaraju; Teresa M. Przytycka; Aaron M. Allen; Stephen F. Goodwin; Cameron W. Berry; Margaret T. Fuller; Helen White‐Cooper

doi:10.1126/science.abk2432

GenomicsScienceMar 3, 2022

Peer-reviewed journal article

Fly Cell Atlas: a single-cell transcriptomic atlas of the adult fruit fly

Hongjie Li iD, Jasper Janssens iD, Maxime De Waegeneer iD, Sai Saroja Kolluru iD, Kristofer Davie iD, Vincent Gardeux iD, Wouter Saelens iD, Fabrice P. A. David iD, Maria Brbić iD, Katina I. Spanier iD, Jure Leskovec iD, Colleen N. McLaughlin iD, Qijing Xie iD, Robert C. Jones iD, Katja Brueckner, Jiwon Shim iD, Sudhir Gopal Tattikota iD, Frank Schnorrer iD, Katja Rust iD, Todd G. Nystul iD, Zita Carvalho-Santos iD, Carlos Ribeiro iD, Soumitra Pal iD, Sharvani Mahadevaraju iD, Teresa M. Przytycka iD, , Kai Li iD

Abstract

The ability to obtain single cell transcriptomes for stable cell types and dynamic cell states is ushering in a new era for biology. We created the Tabula Drosophilae, a single cell atlas of the adult fruit fly which includes 580k cells from 15 individually dissected sexed tissues as well as the entire head and body. Over 100 researchers from the fly community contributed annotations to >250 distinct cell types across all tissues. We provide an in-depth analysis of cell type-related gene signatures and transcription factor markers, as well as sexual dimorphism, across the whole animal. Analysis of common cell types that are shared between tissues, such as blood and muscle cells, allowed the discovery of rare cell types and tissue-specific subtypes. This atlas provides a valuable resource for the entire Drosophila community and serves as a comprehensive reference to study genetic perturbations and disease models at single cell resolution.

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