CRISPR and Genetic Engineering

This paper describes a synthetic CRISPR-Cas nuclease with expanded enzymatic activities, capable of targeting various nucleic acids and functioning in human cells and bacteria.

This paper develops a laboratory model using Drosophila melanogaster to study insecticide susceptibility gene drives, showing they can revert resistance but face challenges like gene drive resistance and population suppression.

The paper attempts to induce Cas9-dependent homologous recombination and gene drive in Tribolium castaneum but finds no evidence of homologous recombination or genetic drive.

The paper introduces a simplified method for amplifying plasmid DNA libraries using rolling circle amplification and endonuclease treatment to improve transformation efficiency in E. coli and S. cerevisiae.

This study corrects a SSBP1 mutation using adenine base editors, improving mitochondrial function but with trade-offs between editing efficacy and off-target risks.

This paper introduces BatCRISPRi, a CRISPRi system in Bacillus subtilis that uses a rapamycin-dependent degron to eliminate leakiness and enable reversible gene knockdown.

The paper introduces a CRISPR-based system called qTAG to improve the efficiency and flexibility of endogenous protein tagging through optimized repair cassettes.

This study shows that nucleotide metabolism limits prime editing efficiency in HSPCs and that deoxynucleoside supplementation and Vpx-mediated SAMHD1 degradation enhance it.

This study introduces RNP-mediated CRISPR/Cas9 for marker-free genome editing in Sporisorium reilianum, achieving efficient mutagenesis through frameshift, knock-in, and knock-out mutants.

The paper compares CRISPR-Cas9 RNP and nickase systems for inducing Aire gene mutations in murine mTECs, finding recurrent mutations with RNP but not nickase.

The paper develops a CRISPR/Cas9 system for Arabidopsis using the NUC1 promoter, achieving high editing efficiency and stable inheritance in the T2 generation.

The paper introduces a method to create specific inversions and rearrangements in fission yeast using CRISPR/Cas9 and a plasmid construction kit.

This study analyzes CRISPR-Cas systems in S. agalactiae genomes, finding two types and their association with phylogenetic clades and anti-CRISPR elements.

This paper develops CRISPR-Cas13 crRNAs that selectively silence oncogenic SNV transcripts with single-base precision by exploiting mismatch intolerance in target-spacer interactions.

The paper develops an effective sgRNA for CRISPR/Cas9-mediated knock-ins in polyploid Synechocystis sp. PCC 6803, enabling reliable genetic modification.

The paper identifies CRIHSP structures in human adaptive immunity genes, showing their association with genomic instability and recombination.

This study uses CRISPR-Cas12a in zebrafish to replicate beta-globin mutations, resulting in amino acid changes but also unintended indels.

The paper introduces casmini-tool, a database for designing dCasMINI guide RNAs with off-target prediction and PAM profile analysis for CRISPRi/a applications.

The paper demonstrates that CRISPR/Cas12a with modified transgenes achieves more efficient gene knock-in in Schistosoma mansoni compared to Cas9.

This paper demonstrates that CRISPR-guided RNA breaks are repaired in human cells, enabling programmable RNA editing to restore gene function.

The paper introduces enGager, a system that enhances ssDNA-mediated genome integration by fusing Cas9 with ssDNA-binding proteins, achieving up to 6-fold efficiency improvements for large transgene knock-in.

Splice Editing uses CRISPR-mediated trans-splicing to correct large RNA segments in human cells, enabling efficient and specific RNA and protein correction without DNA mutagenesis.

This paper introduces qCARMEN, a CRISPR-Cas13-based RNA quantitation method that enables high-throughput, cost-effective analysis of over 4,500 gene-sample pairs, with applications in gene regulation and infection studies.

The paper develops thermoregulated meganucleases to degrade DNA at 18°C, preventing the spread of engineered genetic material outside the mammalian gut.

This paper introduces a method to rewrite large genomic regions using CRISPR/Cas9, enabling simultaneous editing of 7kb+ DNA sections without selection.

The paper uses human genetics and CRISPR/Cas9 to recreate a naturally occurring mutation that increases red blood cell production and shows its combination with a beta-thalassemia correction strategy enhances RBC development.

This study evaluates engineered Cas12a variants for gene inactivation in Xenopus laevis, finding that Cas12a-Ultra achieves high efficiency at low temperatures.

This paper introduces prime-SGE, a prime editing framework for assessing drug resistance variants across multiple oncogenes in a single experiment.

The paper analyzes self-targeting spacers in CRISPR systems and finds they are often associated with membrane proteins, aminoacyl-tRNA synthetases, and ATP-binding proteins.