Erythrocyte Function and Pathophysiology

Gardos potassium channel amplifies PIEZO1-TMEM16F coupling in red blood cells

The paper identifies Gardos as an amplifier of PIEZO1-TMEM16F signaling in red blood cells, enhancing Ca2+ entry and PS externalization, and suggests it as a therapeutic target for diseases like sickle cell disease.

A pooled CRISPR screen reveals genes critical for erythroblast enucleation

This study uses a CRISPR screen to identify genes critical for erythroblast enucleation, finding CLIC3 and VAMP8 as key factors with distinct roles in the process.

The membrane skeleton density of red blood cells in MYH9-related disease patients is decreased

This study finds that MYH9-RD mutations reduce RBC membrane skeleton density and mechanical properties, leading to increased deformation under hyperosmotic stress.

Lipid composition and mechanical force underlie multi-modal regulation of Piezo1 gating

This study shows that Piezo1 channels in cell-derived membranes exhibit mechanical gating and adopt a flattened shape, requiring both mechanical force and a membrane-derived cofactor for activation.

Choice of lipid supplementation for in vitro erythroid cell culture impacts reticulocyte yield and characteristics

This study compares lipid sources in erythroid cultures, finding that detergent-treated plasma leads to cholesterol-deficient reticulocytes with impaired function, while AB-serum supports stable reticulocyte generation.

Metabolic Rigidity as a Mechanical Barrier to Malaria: Flickering Loss in PKLR-Deficient Erythrocytes

This study shows that PKLR-deficient erythrocytes, due to metabolic rigidity, resist Plasmodium yoelii infection by maintaining mechanical properties that hinder parasite-induced membrane changes.

A novel isoform of Tensin1 promotes actin filament assembly for efficient erythroblast enucleation

The paper identifies a novel truncated form of Tensin-1 (eTNS1) that promotes actin filament assembly, essential for efficient erythroblast enucleation during human erythroid differentiation.

Structure of human PIEZO1 and its slow inactivating channelopathy mutants

This study presents cryo-EM structures of human PIEZO1 and its slow-inactivating mutants, revealing structural changes linked to inactivation mechanisms and channelopathies.

Significance of two transmembrane ion gradients for human erythrocyte volume stabilization.

The paper shows that two transmembrane ion gradients and Na/K-ATPase are essential for maintaining human erythrocyte volume stability under increased membrane permeability.

The protein organization of a red blood cell

This study defines the RBC proteome and interactome using mass spectrometry and machine learning, revealing key biological processes and structural models.