Peptidase Inhibition and Analysis

Solid-Phase Synthesis of ProTide Fluorogenic Probes Enables Systematic Profiling of Carboxypeptidase Activity

This study develops fluorogenic ProTide probes to profile carboxypeptidase activities, showing elevated levels in pancreatic cancer patients.

Cryo-electron microscopy reveals a single domain antibody with a unique binding epitope on fibroblast activation protein alpha

The paper reports the cryo-EM structure of a single domain antibody bound to FAP, revealing a unique epitope and describing affinity improvements through CDR3 mutations.

Sensitive quantification of fibroblast activation protein and high-throughput screening for inhibition by FDA-approved compounds

The paper presents a qPCR-based method for high-throughput screening of FDA-approved compounds as FAP inhibitors and demonstrates its application in quantifying FAP in human plasma.

Stabilization of the open conformation of Insulin-Regulated Aminopeptidase by a novel substrate-selective small molecule inhibitor

This paper identifies a nanomolar inhibitor of IRAP that stabilizes its open conformation, revealing a conformation-specific mechanism of action and insights into its catalytic cycle.