This study shows that NAD(H) homeostasis in glycolytic myeloid cells is critical for host protection against tuberculosis, with nicotinamide reducing Mtb lung burden.

bioRxiv · Sep 11, 2022 · PreprintMicrobiology

Mycobacterium tuberculosis senses host Interferon- gamma via the membrane protein MmpL10

M. Ahmed, J. S. Mackenzie, R. Krause, B. Truebody, L. B. Tezera, D. Garay-Baquero, A. F. Vallejo, K. Govender, J. Adamson, P. Elkington, A. J. C. Steyn, A. Leslie

This study shows Mycobacterium tuberculosis senses IFN-γ via MmpL10, increasing respiration and virulence, and enhances INH efficacy against persistent bacteria.

bioRxiv · Nov 12, 2021Microbiology

Host immunity increases Mycobacterium tuberculosis reliance on cytochrome bd oxidase

Y. Cai, E. Jaecklein, J. Mackenzie, K. Papavinasasundaram, A. J. Olive, X. Chen, A. J. C. Steyn, C. M. Sassetti

This study shows that Mycobacterium tuberculosis relies on cytochrome bd oxidase to resist the adaptive immune response, particularly under acidic conditions in macrophages.

bioRxiv · Aug 21, 2020Microbiology

Multiplexed imaging of human tuberculosis granulomas uncovers immunoregulatory features conserved across tissue and blood

E. F. McCaffrey, M. Donato, L. Keren, Z. Chen, M. Fitzpatrick, V. Jojic, A. Delmastro, N. F. Greenwald, A. Baranski, W. Graf, M. Bosse, P. K. Ramdial, E. Forgo, D. Van Valen, M. van de Rijn, , M. Angelo

This study uses multiplexed imaging and transcriptomics to identify immunosuppressive features in TB granulomas and blood, linking local and systemic immune responses.

bioRxiv · Jun 8, 2020 · PreprintImmunology